Description
| Product Name: | Human Flt3 Ligand Recombinant Protein |
| Product Code: | RPPB0257 |
| Size: | 10µg |
| Species: | Human |
| Target: | Flt3 Ligand |
| Synonyms: | Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3. |
| Source: | Baculovirus Sf9 cells |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized with no additives. |
| Solubility: | It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt3-L should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Gln-Asp-Cys-Ser |
| Biological Activity: | The ED50, calculated by the dose-dependent stimulation of the proliferation of human MUTZ-2 cells is < 3.0 ng/ml. |
FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.
Flt3-Ligand Human Recombinant produced in Sf9 insect cells is a glycosylated, polypeptide chain containing 155 amino acids and having migrating on SDS-PAGE at 19kDa. Flt3-Ligand is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | FLT3LG: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytokine; Ligand, receptor tyrosine kinase; Membrane protein, integral Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: extracellular space; membrane; integral to membrane Molecular Function:protein homodimerization activity; cytokine activity; receptor tyrosine kinase binding; receptor binding Biological Process: regulation of myeloid dendritic cell activation; positive regulation of natural killer cell differentiation; lymphocyte differentiation; embryonic hemopoiesis; positive regulation of cell proliferation; positive regulation of protein amino acid phosphorylation; signal transduction; positive regulation of myoblast differentiation |
| NCBI Summary: | Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011] |
| UniProt Code: | P49771 |
| NCBI GenInfo Identifier: | 1706818 |
| NCBI Gene ID: | 2323 |
| NCBI Accession: | P49771.1 |
| UniProt Secondary Accession: | P49771,Q05C96, A0AVC2, B9EGH2, |
| UniProt Related Accession: | P49771 |
| Molecular Weight: | approximately 150 kDa |
| NCBI Full Name: | Fms-related tyrosine kinase 3 ligand |
| NCBI Synonym Full Names: | fms-related tyrosine kinase 3 ligand |
| NCBI Official Symbol: | FLT3LG�� |
| NCBI Official Synonym Symbols: | FL; FLT3L�� |
| NCBI Protein Information: | fms-related tyrosine kinase 3 ligand; flt3 ligand |
| UniProt Protein Name: | Fms-related tyrosine kinase 3 ligand |
| UniProt Synonym Protein Names: | SL cytokine |
| Protein Family: | Receptor-type tyrosine-protein kinase |
| UniProt Gene Name: | FLT3LG�� |
| UniProt Entry Name: | FLT3L_HUMAN |
