Description
| Product Name: | Human Flavokinase Recombinant Protein |
| Product Code: | RPPB2524 |
| Size: | 20µg |
| Species: | Human |
| Target: | Flavokinase |
| Synonyms: | Riboflavin kinase, ATP:riboflavin 5'-phosphotransferase, Flavokinase, RFK, RIFK, FLJ11149, RP11-422N19.2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The Flavokinase solution containing 20mM Tris-HCl buffer (pH8.0) and 10% glycerol. |
| Stability: | Flavokinase although stable at 4°C for 1 week, should be stored below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MPRADCIMRH LPYFCRGQVV RGFGRGSKQL GIPTANFPEQ VVDNLPADIS TGIYYGWASV GSGDVHKMVV SIGWNPYYKN TKKSMETHIM HTFKEDFYGE ILNVAIVGYL RPEKNFDSLE SLISAIQGDI EEAKKRLELP EHLKIKEDNF FQVSKSKIMNGH |
Flavokinase is a transferases family member, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. Flavokinase is an enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), which is an obligatory step in vitamin B2 utilization and flavin cofactor synthesis. It has been proposed that TNF, through the activation of the RFK gene, enhances the incorporation of FAD in NADPH oxidase enzymes, which is a critical step for the assembly and activation of NADPH oxidase.
Flavokinase Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 182 amino acids (1-162 a.a.) and having a molecular mass of 20.5kDa. Flavokinase is fused to 20 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RFK: Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN). |
| UniProt Protein Details: | Protein type:Kinase, other; EC 2.7.1.26; Cofactor and Vitamin Metabolism - riboflavin Chromosomal Location of Human Ortholog: 9q21.13 Cellular Component: cytosol Molecular Function:riboflavin kinase activity Biological Process: apoptosis; positive regulation of NAD(P)H oxidase activity; riboflavin metabolic process |
| NCBI Summary: | Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009] |
| UniProt Code: | Q969G6 |
| NCBI GenInfo Identifier: | 209572667 |
| NCBI Gene ID: | 55312 |
| NCBI Accession: | Q969G6.2 |
| UniProt Secondary Accession: | Q969G6,Q5JSG9, Q9NUT7, |
| UniProt Related Accession: | Q969G6 |
| Molecular Weight: | 17,623 Da |
| NCBI Full Name: | Riboflavin kinase |
| NCBI Synonym Full Names: | riboflavin kinase |
| NCBI Official Symbol: | RFK�� |
| NCBI Official Synonym Symbols: | RIFK�� |
| NCBI Protein Information: | riboflavin kinase |
| UniProt Protein Name: | Riboflavin kinase |
| UniProt Synonym Protein Names: | ATP:riboflavin 5'-phosphotransferase; Flavokinase |
| UniProt Gene Name: | RFK�� |
| UniProt Entry Name: | RIFK_HUMAN |
