Description
| Product Name: | Human FKBP3 Recombinant Protein |
| Product Code: | RPPB1643 |
| Size: | 20µg |
| Species: | Human |
| Target: | FKBP3 |
| Synonyms: | EC 5.2.1.8, FKBP-3, FKBP25, FKBP-25, Peptidyl-prolyl cis-trans isomerase FKBP3, PPIase FKBP3, FK506-binding protein 3, Rotamase, Immunophilin FKBP25, 25 kDa FK506-binding protein, 25 kDa FKBP, Rapamycin-selective 25 kDa immunophilin, FKBP3, PPIase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The FKBP3 protein solution contains 20mM Tris-HCl, pH-8, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MAAAVPQRAW TVEQLRSEQL PKKDIIKFLQ EHGSDSFLAE HKLLGNIKNV AKTANKDHLV TAYNHLFETK RFKGTESISK VSEQVKNVKL NEDKPKETKS EETLDEGPPK YTKSVLKKGD KTNFPKKGDV VHCWYTGTLQ DGTVFDTNIQ TSAKKKKNAK PLSFKVGVGK VIRGWDEALL TMSKGEKARL EIEPEWAYGK KGQPDAKIPP NAKLTFEVEL VDID |
| Biological Activity: | Specific activity is > 490 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin. |
FKBP3 is part of a family of immunosuppressant receptors which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. FKBP3 accelerates the folding of proteins. FKBP3 is part of the immunophilin protein family, which participates in immunoregulation and fundamental cellular processes concerning protein folding and trafficking. FKBP3 is a cis-trans prolyl isomerase enzyme that binds the immunosuppressants FK506 and rapamycin, as well as histone deacetylases, the transcription factor YY1, casein kinase II, and nucleolin. It has a greater affinity for rapamycin than for FK506 and therefore is a significant protein for immunosuppression by rapamycin.
FKBP3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 224 amino acids (1-224 a.a.) and having a molecular mass of 25.1 kDa.FKBP3 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | FKBP3: FK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. PPIases accelerate the folding of proteins. Belongs to the FKBP-type PPIase family. |
| UniProt Protein Details: | Protein type:Isomerase; EC 5.2.1.8 Chromosomal Location of Human Ortholog: 14q21.2 Cellular Component: endoplasmic reticulum membrane; nucleus Molecular Function:protein binding; peptidyl-prolyl cis-trans isomerase activity; FK506 binding; receptor activity Biological Process: protein peptidyl-prolyl isomerization |
| NCBI Summary: | The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin, as well as histone deacetylases, the transcription factor YY1, casein kinase II, and nucleolin. It has a higher affinity for rapamycin than for FK506 and thus may be an important target molecule for immunosuppression by rapamycin. [provided by RefSeq, Sep 2008] |
| UniProt Code: | Q00688 |
| NCBI GenInfo Identifier: | 232096 |
| NCBI Gene ID: | 2287 |
| NCBI Accession: | Q00688.1 |
| UniProt Secondary Accession: | Q00688,Q14317, B2R4Q9, |
| UniProt Related Accession: | Q00688 |
| Molecular Weight: | 224 |
| NCBI Full Name: | Peptidyl-prolyl cis-trans isomerase FKBP3 |
| NCBI Synonym Full Names: | FK506 binding protein 3, 25kDa |
| NCBI Official Symbol: | FKBP3�� |
| NCBI Official Synonym Symbols: | FKBP-3; FKBP25; PPIase; FKBP-25�� |
| NCBI Protein Information: | peptidyl-prolyl cis-trans isomerase FKBP3; rotamase; 25 kDa FKBP; PPIase FKBP3; immunophilin FKBP25; rapamycin binding protein; 25 kDa FK506-binding protein; FK506-binding protein 3 (25kD); FK506-binding protein 25, T-cell; rapamycin-selective 25 kDa immunophilin |
| UniProt Protein Name: | Peptidyl-prolyl cis-trans isomerase FKBP3 |
| UniProt Synonym Protein Names: | 25 kDa FK506-binding protein; 25 kDa FKBP; FKBP-25; FK506-binding protein 3; FKBP-3; Immunophilin FKBP25; Rapamycin-selective 25 kDa immunophilin; Rotamase |
| Protein Family: | FK506-binding protein |
| UniProt Gene Name: | FKBP3�� |
| UniProt Entry Name: | FKBP3_HUMAN |
