Description
| Product Name: | Human FGF 18 Recombinant Protein |
| Product Code: | RPPB0234 |
| Size: | 25µg |
| Species: | Human |
| Target: | FGF 18 |
| Synonyms: | Fibroblast growth factor 18, FGF-18, zFGF5, FGF18. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | FGF-18 protein was lyophilized from a 0.2�m filtered concentrated solution in PBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized FGF-18 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized FGF-18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-18 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | AEENVDFRIH VENQTRARDD VSRKQLRLYQ LYSRTSGKHI QVLGRRISAR GEDGDKYAQL LVETDTFGSQ VRIKGKETEF YLCMNRKGKL VGKPDGTSKE CVFIEKVLEN NYTALMSAKY SGWYVGFTKK GRPRKGPKTR ENQQDVHFMK RYPKGQPELQ KPFKYTTVTK RSRRIRPTHP A� |
| Biological Activity: | The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is�< 0.5ng/ml, corresponding to a specific activity of�> 2.0�106 units/mg. |
Fibroblast growth factor 18 (FGF18) is a member of the large FGF family which has at least 23 members. FGF18 is a binding growth factor with a core 120 amino acid FGF domain which allows for a common tertiary structure. FGFs are expressed in the course of the embryonic development and in restricted adult tissues. FGF-18 is an indispensable regulator of long bone and calvarial development. FGF-18 signals via FGFR 1c, 2c, 3c, and 4.
FGF-18 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 181 amino acids and having a molecular mass of 21.1kDa. The FGF-18 is purified by proprietary chromatographic techniques.�
| UniProt Protein Function: | FGF18: Plays an important role in the regulation of cell proliferation, cell differentiation and cell migration. Required for normal ossification and bone development. Stimulates hepatic and intestinal proliferation. Belongs to the heparin-binding growth factors family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Cytokine; Secreted Chromosomal Location of Human Ortholog: 5q34 Cellular Component: extracellular region; extracellular space; nucleolus Molecular Function:growth factor activity; type 1 fibroblast growth factor receptor binding; type 2 fibroblast growth factor receptor binding Biological Process: activation of MAPKK activity; anatomical structure morphogenesis; angiogenesis; axon guidance; cell-cell signaling; chondrocyte development; endochondral ossification; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; intramembranous ossification; lung development; MAPKKK cascade; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of cell proliferation; positive regulation of chondrocyte differentiation; positive regulation of MAP kinase activity; positive regulation of vascular endothelial growth factor receptor signaling pathway; Ras protein signal transduction; regulation of endothelial cell proliferation; signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway |
| NCBI Summary: | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Knockout studies of the similar gene in mice implied the role of this protein in regulating proliferation and differentiation of midline cerebellar structures. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O76093 |
| NCBI GenInfo Identifier: | 6015149 |
| NCBI Gene ID: | 8817 |
| NCBI Accession: | O76093.1 |
| UniProt Secondary Accession: | O76093,Q6UWF1, D3DQL7, |
| UniProt Related Accession: | O76093 |
| Molecular Weight: | 23,989 Da |
| NCBI Full Name: | Fibroblast growth factor 18 |
| NCBI Synonym Full Names: | fibroblast growth factor 18 |
| NCBI Official Symbol: | FGF18�� |
| NCBI Official Synonym Symbols: | ZFGF5; FGF-18�� |
| NCBI Protein Information: | fibroblast growth factor 18 |
| UniProt Protein Name: | Fibroblast growth factor 18 |
| UniProt Synonym Protein Names: | zFGF5 |
| Protein Family: | Fibroblast growth factor |
| UniProt Gene Name: | FGF18�� |
| UniProt Entry Name: | FGF18_HUMAN |
