Description
| Product Name: | Human FBXO6 Recombinant Protein |
| Product Code: | RPPB3521 |
| Size: | 20µg |
| Species: | Human |
| Target: | FBXO6 |
| Synonyms: | FBG2, FBS2, FBX6, Fbx6b, F-box only protein 6, F-box protein that recognizes sugar chains 2, F-box/G-domain protein 2, FBXO6. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The FBXO6 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMDAPHSK AALDSINELP ENILLELFTH VPARQLLLNC RLVCSLWRDLIDLMTLWKRK CLREGFITKD WDQPVADWKI FYFLRSLHRN LLRNPCAEED MFAWQIDFNG GDRWKVESLPGAHGTDFPDP KVKKYFVTSY EMCLKSQLVD LVAEGYWEEL LDTFRPDIVV KDWFAARADC GCTYQLKVQL ASADYFVLAS FEPPPVTIQQWNNATWTEVS YTFSDYPRGV RYILFQHGGR DTQYWAGWYG PRVTNSSIVV SPKMTRNQAS SEAQPGQKHGQEEAAQSPYR AVVQIF |
F-Box Protein 6, also known as FBXO6, is a part of the F-box protein family which is characterized by a roughly forty amino acid motif, the F-box. The F-box proteins are one of the 4 subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box) that operates in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three categories: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing different protein-protein interaction modules or no recognizable motifs. FBXO6 is a part of the Fbxs category, and its C-terminal area is very similar to that of rat NFB42 (neural F Box 42 kDa) which is involved in the control of the cell cycle.
FBXO6 Human Recombinant produced in E. coli is a single polypeptidechain containing 316 amino acids (1-293) and having a molecular mass of 36.3kDa.�FBXO6 is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.
| UniProt Protein Function: | FBXO6: Substrate-recognition component of some SCF (SKP1-CUL1- F-box protein)-type E3 ubiquitin ligase complexes. Involved in endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranlocated into the cytosol and promoting their ubiquitination and subsequent degradation. Able to recognize and bind denatured glycoproteins, which are modified with not only high-mannose but also complex- type oligosaccharides. Also recognizes sulfated glycans. Also involved in DNA damage response by specifically recognizing activated CHEK1 (phosphorylated on 'Ser-345'), promoting its ubiquitination and degradation. Ubiquitination of CHEK1 is required to insure that activated CHEK1 does not accumulate as cells progress through S phase, or when replication forks encounter transient impediments during normal DNA replication. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 1p36.22 Cellular Component: cytoplasm; cytosol; SCF ubiquitin ligase complex Molecular Function:carbohydrate binding; glycoprotein binding; protein binding; ubiquitin-protein ligase activity Biological Process: DNA damage checkpoint; DNA repair; ER-associated protein catabolic process; glycoprotein catabolic process; protein polyubiquitination; proteolysis; response to unfolded protein; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process |
| NCBI Summary: | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class, and its C-terminal region is highly similar to that of rat NFB42 (neural F Box 42 kDa) which may be involved in the control of the cell cycle. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NRD1 |
| NCBI GenInfo Identifier: | 8922188 |
| NCBI Gene ID: | 26270 |
| NCBI Accession: | NP_060908.1 |
| UniProt Secondary Accession: | Q9NRD1,Q9UKT3, B1AK42, B2RC88, |
| UniProt Related Accession: | Q9NRD1 |
| Molecular Weight: | 33,933 Da |
| NCBI Full Name: | F-box only protein 6 |
| NCBI Synonym Full Names: | F-box protein 6 |
| NCBI Official Symbol: | FBXO6�� |
| NCBI Official Synonym Symbols: | FBG2; FBS2; FBX6; Fbx6b�� |
| NCBI Protein Information: | F-box only protein 6 |
| UniProt Protein Name: | F-box only protein 6 |
| UniProt Synonym Protein Names: | F-box protein that recognizes sugar chains 2; F-box/G-domain protein 2 |
| UniProt Gene Name: | FBXO6�� |
| UniProt Entry Name: | FBX6_HUMAN |
