Description
| Product Name: | Human EYA2 Recombinant Protein |
| Product Code: | RPPB3481 |
| Size: | 10µg |
| Species: | Human |
| Target: | EYA2 |
| Synonyms: | Eyes absent homolog 2 (Drosophila), EAB1, EC 3.1.3.48. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The EYA2 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 40% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMERVFVW DLDETIIIFH SLLTGTFASR YGKDTTTSVR IGLMMEEMIF NLADTHLFFN DLEDCDQIHV DDVSSDDNGQ DLSTYNFSAD GFHSSAPAAN LCLGSGVHGG VDWMRKLAFR YRRVKEMYNT YKNNVGGLIG TPKRETWLQL RAELEALTDL WLTHSLKALN LINSRPNCVN VLVTTTQLIP ALAKVLLYGL GSVFPIENIY SATKTGKESC FERIMQRFGR KAVYVVIGDG VEEEQGAKKH NMPFWRISCH ADLEALRHAL ELEYL |
EYA2 belongs to tyrosine phosphatase family which specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). EYA2 promotes effective DNA mending by dephosphorylating H2AX, promoting the enlistment of DNA repair complexes having MDC1. EYA2 role as histone phosphatase can explain its part in transcription regulation during organogenesis.
EYA2 Human Recombinant produced in E. coli is a single polypeptide chain containing 295 amino acids (244-514) and having a molecular mass of 33.2kDa.EYA2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | EYA2: Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1. Seems to coactivate SIX2, SIX4 and SIX5. Together with SIX1 and DACH2 seem to be involved in myogenesis. May be involved in development of the eye. Interaction with GNAZ and GNAI2 prevents nuclear translocation and transcriptional activity. Belongs to the HAD-like hydrolase superfamily. EYA family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48; Apoptosis; Cell development/differentiation Chromosomal Location of Human Ortholog: 20q13.1 Cellular Component: nucleoplasm Molecular Function:magnesium ion binding; protein binding; protein tyrosine phosphatase activity Biological Process: histone dephosphorylation; mesodermal cell fate specification |
| NCBI Summary: | This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may be post-translationally modified and may play a role in eye development. A similar protein in mice can act as a transcriptional activator. Alternative splicing results in multiple transcript variants, but the full-length natures of all of these variants have not yet been determined. [provided by RefSeq, Jul 2009] |
| UniProt Code: | O00167 |
| NCBI GenInfo Identifier: | 20455483 |
| NCBI Gene ID: | 2139 |
| NCBI Accession: | O00167.2 |
| UniProt Secondary Accession: | O00167,Q5JSW8, Q86U84, Q96CV6, Q96H97, Q99503, Q99812 Q9BWF6, Q9H4S3, Q9H4S9, Q9NPZ4, Q9UIX7, |
| UniProt Related Accession: | O00167 |
| Molecular Weight: | 50,533 Da |
| NCBI Full Name: | Eyes absent homolog 2 |
| NCBI Synonym Full Names: | EYA transcriptional coactivator and phosphatase 2 |
| NCBI Official Symbol: | EYA2�� |
| NCBI Official Synonym Symbols: | EAB1�� |
| NCBI Protein Information: | eyes absent homolog 2 |
| UniProt Protein Name: | Eyes absent homolog 2 |
| Protein Family: | Eyes absent |
| UniProt Gene Name: | EYA2�� |
| UniProt Entry Name: | EYA2_HUMAN |
