The Human Elastase-2 (Neutrophil ELA2) ELISA Kit is a highly reliable tool for the precise measurement of elastase-2 levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Elastase-2, also known as Neutrophil ELA2, is an important enzyme involved in immune response and inflammation. Elevated levels of elastase-2 have been associated with various diseases such as inflammatory conditions, respiratory disorders, and pancreatic diseases.
Understanding the role of elastase-2 in these conditions can provide valuable insights for disease diagnosis and treatment development.By utilizing the Human Elastase-2 (Neutrophil ELA2) ELISA Kit, researchers can effectively study the levels of elastase-2 in different biological samples, leading to a better understanding of its biological functions and potential implications in disease progression. This kit is an essential tool for researchers in the field of immunology, inflammation, and disease research.
Product Name:
Human Elastase 2, Neutrophil (ELA2) ELISA Kit
Product Code:
HUDL00930
Size:
96 Assays
Target:
ELA2
Synonyms:
ELANE, HLE, HNE, NE, PMN-E, Neutrophil Elastase, Medullasin, Polymorphonuclear Leukocyte Elastase, Bone Marrow Serine Protease, Human Leukocyte Elastase, PMN Elastase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
