Description
| Product Name: | Human EIF4E Recombinant Protein |
| Product Code: | RPPB3431 |
| Size: | 10µg |
| Species: | Human |
| Target: | EIF4E |
| Synonyms: | CBP, EIF4E1, EIF4EL1, EIF4F. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The EIF4E 0.5mg/ml protein solution contains 20mM Tris-HCl, pH-8, and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MATVEPETTP TPNPPTTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVEDFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV |
EIF4E is part of the eukaryotic initiation factor 4 families, controls translation of maternal mRNAs in early embryos before the onset of zygotic transcription. EIF4E identifies and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thus modulates the initiation of translation. EIF4E enables ribosome binding by inducing the unwinding of the mRNAs secondary structures.
EIF4E Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 237 amino acids (1-217 a.a.) and having a molecular weight of 27.2kDa. The EIF4E is fused to a 20 aa His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | EIF4E: a protein of the eukaryotic initiation factor 4E family. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the EIF4F complex. |
| UniProt Protein Details: | Protein type:Translation initiation; Translation Chromosomal Location of Human Ortholog: 4q23 Cellular Component: mRNA cap complex; stress granule; eukaryotic translation initiation factor 4F complex; cytoplasm; cytosol Molecular Function:protein binding; eukaryotic initiation factor 4G binding; translation initiation factor activity; RNA cap binding Biological Process: viral reproduction; translation; cytokine and chemokine mediated signaling pathway; positive regulation of mitotic cell cycle; regulation of translation; poly(A) tail shortening; mRNA export from nucleus; cellular protein metabolic process; insulin receptor signaling pathway; translational initiation; gene expression; mRNA catabolic process, deadenylation-dependent decay; G1/S transition of mitotic cell cycle; lung development Disease: Autism, Susceptibility To, 19 |
| NCBI Summary: | The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| UniProt Code: | P06730 |
| NCBI GenInfo Identifier: | 1352435 |
| NCBI Gene ID: | 1977 |
| NCBI Accession: | P06730.2 |
| UniProt Secondary Accession: | P06730,Q96E95, B7Z6V1, D6RCQ6, |
| UniProt Related Accession: | P06730 |
| Molecular Weight: | 27,260 Da |
| NCBI Full Name: | Eukaryotic translation initiation factor 4E |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 4E |
| NCBI Official Symbol: | EIF4E�� |
| NCBI Official Synonym Symbols: | CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1�� |
| NCBI Protein Information: | eukaryotic translation initiation factor 4E; eIF-4E; eIF-4F 25 kDa subunit; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein |
| UniProt Protein Name: | Eukaryotic translation initiation factor 4E |
| UniProt Synonym Protein Names: | eIF-4F 25 kDa subunit; mRNA cap-binding protein |
| Protein Family: | Eukaryotic translation initiation factor |
| UniProt Gene Name: | EIF4E�� |
| UniProt Entry Name: | IF4E_HUMAN |
