Description
| Product Name: | Human EGFL6 Recombinant Protein |
| Product Code: | RPPB0173 |
| Size: | 10µg |
| Species: | Human |
| Target: | EGFL6 |
| Synonyms: | EGF Like Domain Multiple 6, MAM and EGF Domains-Containing Gene Protein, MAM and EGF Domain Containing, EGF-Like Protein 6, MAEG, EGF Repeat-Containing Protein 6, W80, EGFL6. |
| Source: | HEK293 Cells |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The EGFL6 protein was lyophilized from a 0.2�m filtered solution in 20mM MES and 500mM NaCl, pH 6.0 with 5% Trehalose. |
| Solubility: | It is recommended to reconstitute the lyophilized EGFL6 in sterile PBS at 500�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized EGFL6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGFL6 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Biological Activity: | EGFL6 activity is determined by its ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. The expected ED50 for this effect is 1-5 ?g/ml. |
Epidermal Growth Factor�like Domain Multiple 6 (EGFL6) belongs to the EGF repeat superfamily of proteins, whose members are involved in the regulation of cell cycle, proliferation, and developmental processes. EGFL6 gene product contains a signal peptide, suggesting that EGFL6 is secreted; an EGF repeat region consisting of four complete EGF-like repeats and 1 partial EGF-like repeat, 3 of which have a calcium-binding consensus sequence; an arg-gly-asp integrin association motif; and a MAM domain, which is assumed to have an adhesive function. Within shared regions, human EGFL6 shares 75% and 78% amino acid sequence identity with the mouse and rat orthologs, respectively. EGFL6 is expressed in various fetal tissues during early development such as the lung, heart, liver, spleen, cochlea and the placenta, as well as meningioma tumors.
EGF Like Domain Multiple 6 Human Recombinant produced in HEK cells is a polypeptide chain starting at amino acid Asn at position 22 to amino acid Arg at position 363, fused to an FC, 6 x His-tag at C-terminus, containing a total of 348 amino acids and having a predicted molecular mass of 40-55kDa. The EGFL6 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | EGFL6: May bind integrin alpha-8/beta-1 and play a role in hair follicle morphogenesis. Promotes matrix assembly. Belongs to the nephronectin family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Secreted, signal peptide; SecretedChromosomal Location of Human Ortholog: Xp22Cellular Component: extracellular space; membrane; basement membraneMolecular Function: integrin binding; calcium ion bindingBiological Process: extracellular matrix organization and biogenesis; multicellular organismal development; cell cycle; cell adhesion; cell differentiation |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the epidermal growth factor (EGF) repeat superfamily. Members of this superfamily are characterized by the presence of EGF-like repeats and are often involved in the regulation of cell cycle, proliferation, and developmental processes. The gene product contains a signal peptide, suggesting that it is secreted; an EGF repeat region consisting of 4 complete EGF-like repeats and 1 partial EGF-like repeat, 3 of which have a calcium-binding consensus sequence; an arg-gly-asp integrin association motif; and a MAM domain, which is believed to have an adhesive function. This gene is expressed early during development, and its expression has been detected in lung and meningioma tumors. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8IUX8 |
| NCBI GenInfo Identifier: | 268607667 |
| NCBI Gene ID: | 25975 |
| NCBI Accession: | NP_001161362.1 |
| UniProt Secondary Accession: | Q8IUX8,Q6UXJ1, Q8NBV0, Q8WYG3, Q9NY67, Q9NZL7, Q9UFK6 B2RCB1 |
| UniProt Related Accession: | Q8IUX8 |
| Molecular Weight: | 61,388 Da |
| NCBI Full Name: | epidermal growth factor-like protein 6 isoform 2 |
| NCBI Synonym Full Names: | EGF-like-domain, multiple 6 |
| NCBI Official Symbol: | EGFL6�� |
| NCBI Official Synonym Symbols: | W80; MAEG�� |
| NCBI Protein Information: | epidermal growth factor-like protein 6 |
| UniProt Protein Name: | Epidermal growth factor-like protein 6 |
| UniProt Synonym Protein Names: | MAM and EGF domains-containing gene protein |
| Protein Family: | Epidermal growth factor-like protein |
| UniProt Gene Name: | EGFL6�� |
| UniProt Entry Name: | EGFL6_HUMAN |
