Description
| Product Name: | Human EG VEGF Recombinant Protein |
| Product Code: | RPPB0161 |
| Size: | 20µg |
| Species: | Human |
| Target: | EG VEGF |
| Synonyms: | PK1, PRK1, Prokineticin 1, EG-VEGF. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized from a concentrated (1mg/ml) solution containing 0.1% Trifluoroacetic Acid (TFA). |
| Solubility: | It is recommended to reconstitute the lyophilized Endocrine Gland Vascular Endothelial Growth Factor in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized EG-VEGF Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EG-VEGF should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF |
| Biological Activity: | The activity as determined by the dose-dependent proliferation of MIA PaCa-2 cells is typically 1-4 ?g/ml. |
Endocrine gland-derived vascular endothelial growth factor (EG-VEGF) induces proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. Its expression is induced by hypoxia and is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta). Its expression is often complementary to the expression of VEGF (MIM 192240), suggesting that these molecules function in a coordinated manner. EG-VEGF potently contracts gastrointestinal (gi) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types.
EG-VEGF Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 86 amino acids and having a molecular mass of 9.7kDa. The EG-VEGF is purified by proprietary chromatographic techniques.�
| UniProt Protein Function: | PROK1: Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis. Belongs to the AVIT (prokineticin) family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide; Cell cycle regulation Chromosomal Location of Human Ortholog: 1p21 Cellular Component: extracellular region Molecular Function:growth factor activity Biological Process: activation of MAPK activity; positive regulation of cell division; positive regulation of cell proliferation; angiogenesis; regulation of angiogenesis |
| NCBI Summary: | The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. [provided by RefSeq, Sep 2011] |
| UniProt Code: | P58294 |
| NCBI GenInfo Identifier: | 17380189 |
| NCBI Gene ID: | 84432 |
| NCBI Accession: | P58294.1 |
| UniProt Secondary Accession: | P58294,Q5VWD4, Q8TC69, |
| UniProt Related Accession: | P58294 |
| Molecular Weight: | 11,715 Da |
| NCBI Full Name: | Prokineticin-1 |
| NCBI Synonym Full Names: | prokineticin 1 |
| NCBI Official Symbol: | PROK1�� |
| NCBI Official Synonym Symbols: | PK1; PRK1; EGVEGF�� |
| NCBI Protein Information: | prokineticin-1; EG-VEGF; mambakine; black mamba toxin-related protein; endocrine-gland-derived vascular endothelial growth factor |
| UniProt Protein Name: | Prokineticin-1 |
| UniProt Synonym Protein Names: | Endocrine-gland-derived vascular endothelial growth factor; EG-VEGF; Mambakine |
| Protein Family: | Prokineticin |
| UniProt Gene Name: | PROK1�� |
| UniProt Entry Name: | PROK1_HUMAN |
