Description
| Product Name: | Human EEF1E1 Recombinant Protein |
| Product Code: | RPPB3397 |
| Size: | 20µg |
| Species: | Human |
| Target: | EEF1E1 |
| Synonyms: | Eukaryotic translation elongation factor 1 epsilon-1, Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, Elongation factor p18, Multisynthase complex auxiliary component p18, EEF1E1, AIMP3, P18. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The EEF1E1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol 0.1M NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAAAAELSLL EKSLGLSKGN KYSAQGERQI PVLQTNNGPS LTGLTTIAAH LVKQANKEYL LGSTAEEKAI VQQWLEYRVT QVDGHSSKND IHTLLKDLNS YLEDKVYLTG YNFTLADILL YYGLHRFIVD LTVQEKEKYL NVSRWFCHIQ HYPGIRQHLS SVVFIKNRLY TNSH |
Eukaryotic translation elongation factor 1 epsilon-1 (EEF1E1) shares sequence similarity with the amino-terminal ends of the Beta and Gamma subunits of EF-1. By specifically interacting with MetRS, EEF1E1 binds to a macromolecular tRNA synthtase complex which catalyzes the ligation of specific amino acids to their cognate tRNAs. Upon DNA damage, EEF1E1 translocates to the nucleus where it interacts with ATM and ATR, resulting in p53 activation.
EEF1E1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 194 amino acids (1-174 a.a.) and having a molecular mass of 21.9kDa.EEF1E1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | EEF1E1: Positive modulator of ATM response to DNA damage. |
| UniProt Protein Details: | Protein type:Translation; Translation elongation Chromosomal Location of Human Ortholog: 6p24.3 Cellular Component: cytoplasm; nucleus; cytosol Molecular Function:protein binding Biological Process: negative regulation of cell proliferation; tRNA aminoacylation for protein translation; positive regulation of apoptosis; gene expression; positive regulation of DNA damage response, signal transduction by p53 class mediator |
| NCBI Summary: | This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2010] |
| UniProt Code: | O43324 |
| NCBI GenInfo Identifier: | 17378996 |
| NCBI Gene ID: | 9521 |
| NCBI Accession: | O43324.1 |
| UniProt Related Accession: | O43324 |
| Molecular Weight: | 20; 16; 20kDa |
| NCBI Full Name: | Eukaryotic translation elongation factor 1 epsilon-1 |
| NCBI Synonym Full Names: | eukaryotic translation elongation factor 1 epsilon 1 |
| NCBI Official Symbol: | EEF1E1�� |
| NCBI Official Synonym Symbols: | P18; AIMP3�� |
| NCBI Protein Information: | eukaryotic translation elongation factor 1 epsilon-1 |
| UniProt Protein Name: | Eukaryotic translation elongation factor 1 epsilon-1 |
| UniProt Synonym Protein Names: | Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3; Elongation factor p18; Multisynthase complex auxiliary component p18 |
| Protein Family: | Eukaryotic translation elongation factor |
| UniProt Gene Name: | EEF1E1�� |
| UniProt Entry Name: | MCA3_HUMAN |
