Description
| Product Name: | Human EEF1D Recombinant Protein |
| Product Code: | RPPB3396 |
| Size: | 10µg |
| Species: | Human |
| Target: | EEF1D |
| Synonyms: | Elongation factor 1-delta, EF-1-delta, Antigen NY-CO-4, EEF1D, EF1D, EF-1D, FP1047, FLJ20897. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The EEF1D solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MATNFLAHEK IWFDKFKYDD AERRFYEQMN GPVAGASRQE NGASVILRDI ARARENIQKS LAGSSGPGAS SGTSGDHGEL VVRIASLEVE NQSLRGVVQE LQQAISKLEA RLNVLEKSSP GHRATAPQTQ HVSPMRQVEP PAKKPATPAE DDEDDDIDLF GSDNEEEDKE AAQLREERLR QYAEKKAKKP ALVAKSSILL DVKPWDDETD MAQLEACVRS IQLDGLVWGA SKLVPVGYGI RKLQIQCVVE DDKVGTDLLE EEITKFEEHV QSVDIAAFNK I |
Eukaryotic translation elongation factor 1 delta (EEF1D) is a subunit of the elongation factor-1 complex that is responsible for the enzymatic transport of aminoacyl tRNAs to the ribosome. EEF1D functions as guanine nucleotide exchange factor. EEF1D interacts with HIV-1 Tat, and consequently represses the translation of host-cell, but not HIV-1, mRNAs. EEF1D overexpression is linked to oesophageal carcinoma and may negatively influence the outcome of medulloblastomas.
EEF1D Human Recombinant fused with a�20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing�301 amino acids (1-281 a.a.) and having a molecular mass of 33.2kDa (Molecular weight on SDS-PAGE will appear higher). The EEF1D is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | EEF1D: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. Belongs to the EF-1-beta/EF-1-delta family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Translation elongation; Translation Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: eukaryotic translation elongation factor 1 complex; endoplasmic reticulum; cytoplasm; nucleolus; nucleus; cytosol Molecular Function:signal transducer activity; protein binding; transcription activator binding; DNA binding; translation factor activity, nucleic acid binding; heat shock protein binding; translation elongation factor activity Biological Process: cellular protein metabolic process; translational elongation; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of transcription, DNA-dependent; translation; transcription, DNA-dependent; mRNA transcription; gene expression; signal transduction |
| NCBI Summary: | This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010] |
| UniProt Code: | P29692 |
| NCBI GenInfo Identifier: | 20141357 |
| NCBI Gene ID: | 1936 |
| NCBI Accession: | P29692.5 |
| UniProt Secondary Accession: | P29692,Q4VBZ6, Q969J1, Q96I38, B4DDU4, D3DWK3, E9PBQ9 |
| UniProt Related Accession: | P29692 |
| Molecular Weight: | 281 |
| NCBI Full Name: | Elongation factor 1-delta |
| NCBI Synonym Full Names: | eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) |
| NCBI Official Symbol: | EEF1D�� |
| NCBI Official Synonym Symbols: | EF1D; EF-1D; FP1047�� |
| NCBI Protein Information: | elongation factor 1-delta; EF-1-delta; antigen NY-CO-4; guanine nucleotide exchange protein |
| UniProt Protein Name: | Elongation factor 1-delta |
| UniProt Synonym Protein Names: | Antigen NY-CO-4 |
| Protein Family: | Elongation factor |
| UniProt Gene Name: | EEF1D�� |
| UniProt Entry Name: | EF1D_HUMAN |
