Description
| Product Name: | Human EDF1 Recombinant Protein |
| Product Code: | RPPB3392 |
| Size: | 25µg |
| Species: | Human |
| Target: | EDF1 |
| Synonyms: | EDF-1, MBF1, Multiprotein-bridging factor 1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | 0.5 mg/ml solution containing 20mM Tris pH-8, 1mM DTT, 0.1M NaCl & 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK QHSITKNTAK LDRETEELHH DRVTLEVGKV IQQGRQSKGL TQKDLATKIN EKPQVIADYE SGRAIPNNQV LGKIERAIGL KLRGKDIGKP IEKGPRAKLE HHHHHH |
EDF1 controls endothelial cell differentiation. EDF1 has a role as a bridging protein that interconnects regulatory proteins and the basal transcriptional machinery, thus modulating the transcription of genes that take part in endothelial differentiation. EDF1 binds calmodulin through its IQ domain and controls nitric oxide synthase activity via calmodulin sequestration in the cytoplasm. EDF1 is localized in adult liver, heart, adipose tissues, intestine and pancreas.
EDF1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 156 amino acids (1-148 a.a.) and having a molecular mass of 17.4 kDa. The EDF1 is fused to 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | EDF1: Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; DNA-binding; Nuclear receptor co-regulator; RNA-binding Chromosomal Location of Human Ortholog: 9q34.3 Cellular Component: transcription factor TFIID complex; cytoplasm; nucleolus; intracellular; nucleus Molecular Function:calmodulin binding; methyltransferase activity; protein binding; histone acetyltransferase activity; sequence-specific DNA binding; transcription coactivator activity; transcription factor activity Biological Process: endothelial cell differentiation; regulation of lipid metabolic process; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of DNA binding |
| UniProt Code: | O60869 |
| NCBI GenInfo Identifier: | 62510569 |
| NCBI Gene ID: | |
| NCBI Accession: | O60869.1 |
| Molecular Weight: | ~ 16kDa |
| NCBI Full Name: | Endothelial differentiation-related factor 1 |
| UniProt Protein Name: | Endothelial differentiation-related factor 1 |
| UniProt Synonym Protein Names: | Multiprotein-bridging factor 1; MBF1 |
| Protein Family: | Endothelial differentiation-related factor |
| UniProt Gene Name: | EDF1�� |
| UniProt Entry Name: | EDF1_HUMAN |
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