Description
| Product Name: | Human DPPA3 Recombinant Protein |
| Product Code: | RPPB3371 |
| Size: | 10µg |
| Species: | Human |
| Target: | DPPA3 |
| Synonyms: | Developmental pluripotency-associated protein 3, Stella-related protein, DPPA3, STELLAR, STELLA. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | DPPA3 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMDPSQFN PTYIPGSPQM LTEENSRDDS GASQISSETL IKNLSNLTIN ASSESVSPLS EALLRRESVG AAVLREIEDE WLYSRRGVRT LLSVQREKMA RLRYMLLGGV RTHERRPTNK EPKGVKKESR PFKCPCSFCV SNGWDPSENA RIGNQDTKPL QP |
Developmental Pluripotency Associated 3 (DPPA3) is a protein which in mice may serve as a maternal factor during the preimplantation stage of development. In mice, the DPPA3 protein has a role in transcriptional repression, cell division, and maintenance of cell pluripotentiality. In humans, related intronless loci are found on chromosomes 14 and X. DPPA3 is expressed in embryonic stem and carcinoma cells. DPPA3 has a low expression in the testis, ovary and thymus. DPPA3 is highly expressed in testicular germ cell tumors.
DPPA3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 182 amino acids (1-159 a.a) and having a molecular mass of 20.2kDa.DPPA3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DPPA3: Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis.Chromosomal Location of Human Ortholog: 12p13.31Cellular Component: cytoplasm; female pronucleus; male pronucleus; nucleusMolecular Function: methylated histone residue binding |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a protein that in mice may function as a maternal factor during the preimplantation stage of development. In mice, this gene may play a role in transcriptional repression, cell division, and maintenance of cell pluripotentiality. In humans, related intronless loci are located on chromosomes 14 and X. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q6W0C5 |
| NCBI GenInfo Identifier: | 74749483 |
| NCBI Gene ID: | 359787 |
| NCBI Accession: | Q6W0C5.1 |
| UniProt Secondary Accession: | Q6W0C5,Q0P5U3, Q6JZS6 |
| UniProt Related Accession: | Q6W0C5 |
| Molecular Weight: | 17,851 Da |
| NCBI Full Name: | Developmental pluripotency-associated protein 3 |
| NCBI Synonym Full Names: | developmental pluripotency associated 3 |
| NCBI Official Symbol: | DPPA3�� |
| NCBI Official Synonym Symbols: | STELLA�� |
| NCBI Protein Information: | developmental pluripotency-associated protein 3 |
| UniProt Protein Name: | Developmental pluripotency-associated protein 3 |
| UniProt Synonym Protein Names: | Stella-related protein |
| Protein Family: | Developmental pluripotency-associated protein |
| UniProt Gene Name: | DPPA3�� |
| UniProt Entry Name: |
