Description
| Product Name: | Human DPH2 Recombinant Protein |
| Product Code: | RPPB3369 |
| Size: | 5µg |
| Species: | Human |
| Target: | DPH2 |
| Synonyms: | DPH2 Homolog, S-Adenosyl-L-Methionine:L-Histidine 3-Amino-3-Carboxypropyltransferase 2, Diphtheria Toxin Resistance Protein 2, Diphthamide Biosynthesis Protein 2, DPH2L2, Diptheria Toxin Resistance Protein Required For Diphthamide Biosynthesis-Like 2, 2-(3-Amino-3-Carboxypropyl)Histidine Synthase Subunit 2, Diphthamide Biosynthesis Protein 2 Homolog-Like 2, Diphthamide Biosynthesis-Like Protein 2, EC 2.5.1.108, DPH2-Like 2, DPH2. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | DPH2 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4), 1mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MESMFSSPAE AALQRETGVP GLLTPLPDLD GVYELERVAG FVRDLGCERV ALQFPDQLLG DAVAVAARLE ETTGSKMFIL GDTAYGSCCV DVLGAEQAGA QALIHFGPAC LSPPARPLPV AFVLRQRSVA LELCVKAFEA QNPDPKAPVV LLSEPACAHA LEALATLLRP RYLDLLVSSP AFPQPVGSLS PEPMPLERFG RRFPLAPGRR LEEYGAFYVG GSKASPDPDL DPDLSRLLLG WAPGQPFSSC CPDTGKTQDE GARAGRLRAR RRYLVERARD ARVVGLLAGT LGVAQHREAL AHLRNLTQAA GKRSYVLALG RPTPAKLANF PEVDVFVLLA CPLGALAPQL SGSFFQPILA PCELEAACNP AWPPPGLAPH LTHYADLLPG SPFHVALPPP ESELWETPDV SLITGDLRPP PAWKSSNDHG SLALTPRPQL ELAESSPAAS FLSSRSWQGL EPRLGQTPVT EAVSGRRGIA IAYEDEGSGL EHHHHHH |
Diphthamide biosynthesis protein 2 (DPH2) is a homodimer and each of its monomers can bind a [4Fe-4S] cluster. DPH2 is the target of ADP ribosylating diphtheria toxin (DT) and Pseudomonas exotoxin A (PE). DPH2 was identified by its ability to complement a diphthamide mutant strain, and thus serves in diphthamide biosynthesis. The loss of DPH2 pre-activates NF-kB and death receptor pathways and renders MCF7 cells hypersensitive to tumor necrosis factor.�
DPH2 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 497 amino acids (1-489a.a.) and having a molecular mass of 53.1kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). DPH2 is expressed with an 8 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). |
| NCBI Summary: | This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016] |
| UniProt Code: | Q9BQC3 |
| NCBI GenInfo Identifier: | 74761201 |
| NCBI Gene ID: | 1802 |
| NCBI Accession: | Q9BQC3.1 |
| UniProt Secondary Accession: | Q9BQC3,O60623, A8MVC9, B2RDE3, B4DNI8, |
| UniProt Related Accession: | Q9BQC3 |
| Molecular Weight: | 38,510 Da |
| NCBI Full Name: | 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 |
| NCBI Synonym Full Names: | DPH2 homolog |
| NCBI Official Symbol: | DPH2�� |
| NCBI Official Synonym Symbols: | DPH2L2�� |
| NCBI Protein Information: | 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2; diphthamide biosynthesis protein 2 |
| UniProt Protein Name: | 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 |
| UniProt Synonym Protein Names: | Diphthamide biosynthesis protein 2; Diphtheria toxin resistance protein 2Curated; S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 2Curated |
| Protein Family: | 2-(3-amino-3-carboxypropyl)histidine synthase |
| UniProt Gene Name: | DPH2�� |
