Description
| Product Name: | Human DNAJC24 Recombinant Protein |
| Product Code: | RPPB5208 |
| Size: | 10µg |
| Species: | Human |
| Target: | DNAJC24 |
| Synonyms: | DnaJ homolog subfamily C member 24, DNAJC24, DPH4, JJJ3, ZCSL3, CSL-type zinc finger-containing protein 3, Diphthamide biosynthesis protein 4. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The DNAJC24 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMMAVEQM PKKDWYSILG ADPSANISDL KQKYQKLILM YHPDKQSTDV PAGTVEECVQ KFIEIDQAWK ILGNEETKRE YDLQRCEDDL RNVGPVDAQV YLEEMSWNEG DHSFYLSCRC GGKYSVSKDE AEEVSLISCD TCSLIIELLH YN |
DnaJ homolog subfamily C member 24 (DNAJC24) belongs to the ZCSL family of proteins and each contain one DPH-type zinc finger. DNAJC24 is involved in the synthesis of diphthamide, a protein found on translation elongation factor EF-2 which is the target of bacterial ADP-ribosylating toxins. DNAJC24 stimulates the ATPase activity of few Hsp70-type chaperones. DNAJC24 detected in heart, brain, spleen, lung, liver, kidney and testis.
DNAJC24 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 172 amino acids (1-149 a.a.) and having a molecular mass of 19.5kDa. DNAJC24 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DNAJC24: Stimulates the ATPase activity of several Hsp70-type chaperones. Belongs to the DPH4 family. |
| UniProt Protein Details: | Protein type:Enzyme, misc.; Chaperone Chromosomal Location of Human Ortholog: 11p13 Molecular Function:ATPase activator activity; ferrous iron binding; zinc ion binding Biological Process: positive regulation of ATPase activity |
| NCBI Summary: | Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q6P3W2 |
| NCBI GenInfo Identifier: | 66773991 |
| NCBI Gene ID: | 120526 |
| NCBI Accession: | Q6P3W2.1 |
| UniProt Secondary Accession: | Q6P3W2,A8K0V0, B1ALC1, I6L9B4, |
| UniProt Related Accession: | Q6P3W2 |
| Molecular Weight: | 9,693 Da |
| NCBI Full Name: | DnaJ homolog subfamily C member 24 |
| NCBI Synonym Full Names: | DnaJ heat shock protein family (Hsp40) member C24 |
| NCBI Official Symbol: | DNAJC24�� |
| NCBI Official Synonym Symbols: | DPH4; JJJ3; ZCSL3�� |
| NCBI Protein Information: | dnaJ homolog subfamily C member 24 |
| UniProt Protein Name: | DnaJ homolog subfamily C member 24 |
| UniProt Synonym Protein Names: | CSL-type zinc finger-containing protein 3; Diphthamide biosynthesis protein 4 |
| Protein Family: | DnaJ homolog subfamily |
| UniProt Gene Name: | DNAJC24�� |
| UniProt Entry Name: | DJC24_HUMAN |
