Description
| Product Name: | Human DFFA Recombinant Protein |
| Product Code: | RPPB3350 |
| Size: | 25µg |
| Species: | Human |
| Target: | DFFA |
| Synonyms: | DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, DFF-45, Inhibitor of CAD, ICAD, DFFA, DFF1, DFF45. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The DFFA solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL AIDKSLTPVT LVLAEDGTIV DDDDYFLCLP SNTKFVALAS NEKWAYNNSD GGTAWISQES FDVDETDSGA GLKWKNVARQ LKEDLSSIIL LSEEDLQMLV DAPCSDLAQE LRQSCATVQR LQHTLQQVLD QREEVRQSKQ LLQLYLQALE KEGSLLSKQE ESKAAFGEEV DAVDTGISRE TSSDVALASH ILTALREKQA PELSLSSQDL ELVTKEDPKA LAVALNWDIK KTETVQEACE WELALRLQQT QSLHSLRSIS ASKASPPGDL QNPKRARQDP T |
DFF is a heterodimeric protein of 40kDa (DFFB) and 45kDa (DFFA) subunits. DFFA (DNA fragmentation factor subunit alpha) is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF is activated once DFFA is cleaved by caspase-3. The cleaved fragments of DFFA detach from DFFB (the active component of DFF), which in turn triggers DNA fragmentation as well as chromatin condensation during apoptosis. Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. A reduced level of DFFA detected in ovarian endometriosis may be a part of an apoptosis-resistant mechanism enhancing the disease progression.DFFA at chromosome 1 shows rare allelic variants in neuroblastoma tumors.
DFFA Human Recombinant fused with 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 351 amino acids (1- 331 a.a.) and having a molecular mass of 38.7kDa.The DFFA is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DFFA: Inhibitor of the caspase-activated DNase (DFF40). Heterodimer of DFFA and DFFB. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Hydrolase Chromosomal Location of Human Ortholog: 1p36.22 Cellular Component: cytosol; lipid particle; nuclear chromatin; nucleoplasm; nucleus; plasma membrane Molecular Function:protein binding; protein domain specific binding Biological Process: DNA fragmentation during apoptosis; negative regulation of deoxyribonuclease activity |
| NCBI Summary: | Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O00273 |
| NCBI GenInfo Identifier: | 2810997 |
| NCBI Gene ID: | 1676 |
| NCBI Accession: | O00273.1 |
| UniProt Secondary Accession: | O00273,Q5T6G5, Q5T6G6, Q96I97, Q9Y6C6, |
| UniProt Related Accession: | O00273 |
| Molecular Weight: | 37kDa |
| NCBI Full Name: | DNA fragmentation factor subunit alpha |
| NCBI Synonym Full Names: | DNA fragmentation factor subunit alpha |
| NCBI Official Symbol: | DFFA�� |
| NCBI Official Synonym Symbols: | DFF1; ICAD; DFF-45�� |
| NCBI Protein Information: | DNA fragmentation factor subunit alpha |
| UniProt Protein Name: | DNA fragmentation factor subunit alpha |
| UniProt Synonym Protein Names: | DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD |
| Protein Family: | DNA fragmentation factor |
| UniProt Gene Name: | DFFA�� |
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