Description
| Product Name: | Human DARS Recombinant Protein |
| Product Code: | RPPB1554 |
| Size: | 20µg |
| Species: | Human |
| Target: | DARS |
| Synonyms: | Aspartyl-tRNA synthetase, Cell proliferation-inducing gene 40 protein, AspRS, aspartate tRNA ligase 1 cytoplasmic, EC 6.1.1.12. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The DARS solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 150mM Nacl, 1mM DTT and 40% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MPSASASRKS QEKPREIMDA AEDYAKERYG ISSMIQSQEK PDRVLVRVRD LTIQKADEVV WVRARVHTSR AKGKQCFLVL RQQQFNVQAL VAVGDHASKQ MVKFAANINK ESIVDVEGVV RKVNQKIGSC TQQDVELHVQ KIYVISLAEP RLPLQLDDAV RPEAEGEEEG RATVNQDTRL DNRVIDLRTS TSQAVFRLQS GICHLFRETL INKGFVEIQT PKIISAASEG GANVFTVSYF KNNAYLAQSP QLYKQMCICA DFEKVFSIGP VFRAEDSNTH RHLTEFVGLD IEMAFNYHYH EVMEEIADTM VQIFKGLQER FQTEIQTVNK QFPCEPFKFL EPTLRLEYCE ALAMLREAGV EMGDEDDLST PNEKLLGHLV KEKYDTDFYI LDKYPLAVRP FYTMPDPRNP KQSNSYDMFM RGEEILSGAQ RIHDPQLLTE RALHHGIDLE KIKAYIDSFR FGAPPHAGGG IGLERVTMLF LGLHNVRQTS MFPRDPKRLT P |
DARS uses a 2 step reaction to catalyze the specific attachment of an amino acid to its cognate tRNA: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
DARS Recombinant produced in E. coli is a single polypeptide chain containing 521 amino acids (1-501) and having a molecular mass of 59.3kDa.DARS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Belongs to the class-II aminoacyl-tRNA synthetase family. |
| UniProt Protein Details: | Protein type:Translation; EC 6.1.1.12; Ligase Chromosomal Location of Human Ortholog: 2q21.3 Cellular Component: membrane; cytoplasm; cytosol Molecular Function:protein binding; aspartate-tRNA ligase activity; ATP binding; aminoacylase activity Biological Process: tRNA aminoacylation for protein translation; translation; protein complex assembly; gene expression; aspartyl-tRNA aminoacylation Disease: Hypomyelination With Brainstem And Spinal Cord Involvement And Leg Spasticity |
| NCBI Summary: | This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] |
| UniProt Code: | P14868 |
| NCBI GenInfo Identifier: | 20178330 |
| NCBI Gene ID: | 1615 |
| NCBI Accession: | P14868.2 |
| UniProt Secondary Accession: | P14868,Q2TNI3, Q32Q69, Q53HV4, Q53YC5, Q68CR9, Q9BW52 A8K3J2, D3DP77, |
| UniProt Related Accession: | P14868 |
| Molecular Weight: | 501 |
| NCBI Full Name: | Aspartate--tRNA ligase, cytoplasmic |
| NCBI Synonym Full Names: | aspartyl-tRNA synthetase |
| NCBI Official Symbol: | DARS�� |
| NCBI Official Synonym Symbols: | HBSL; aspRS�� |
| NCBI Protein Information: | aspartate--tRNA ligase, cytoplasmic; aspartate tRNA ligase 1, cytoplasmic; aspartyl-tRNA synthetase, cytoplasmic; cell proliferation-inducing gene 40 protein |
| UniProt Protein Name: | Aspartate--tRNA ligase, cytoplasmic |
| UniProt Synonym Protein Names: | Aspartyl-tRNA synthetase; AspRS; Cell proliferation-inducing gene 40 protein |
| UniProt Gene Name: | DARS�� |
| UniProt Entry Name: | SYDC_HUMAN |
