Description
| Product Name: | Human DAP Recombinant Protein |
| Product Code: | RPPB3326 |
| Size: | 10µg |
| Species: | Human |
| Target: | DAP |
| Synonyms: | Death-associated protein 1, DAP-1, DAP, DAP1, Death-Associated Protein. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | DAP protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles. |
| Purity: | Greaterthan 80% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMSSPPEG KLETKAGHPP AVKAGGMRIV QKHPHTGDTK EEKDKDDQEW ESPSPPKPTV FISGVIARGD KDFPPAAAQV AHQKPHASMD KHPSPRTQHI QQPRK |
Death-Associated Protein (DAP) is a proline-rich protein. DAP, which is a negative regulator of autophagy, takes part in the mediation of IFN-gamma-induced cell death.
DAP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 125 amino acids (1-102 a.a.) and having a molecular mass of 13.6kDa.DAP is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DAP: a 15 kDa, proline rich protein that possesses one SH3 binding motif. Negatively regulates autophagy and is involved in mediating interferon-gamma-induced cell death. Localizes in the cytoplasm, but the detailed mechanism of its proapoptotic function is unclear. |
| UniProt Protein Details: | Protein type:Autophagy Chromosomal Location of Human Ortholog: 5p15.2 Biological Process: apoptosis; caspase activation; cellular response to amino acid starvation; inhibition of NF-kappaB transcription factor; negative regulation of autophagy; negative regulation of transcription, DNA-dependent |
| NCBI Summary: | This gene encodes a basic, proline-rich, 15-kD protein. The protein acts as a positive mediator of programmed cell death that is induced by interferon-gamma. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014] |
| UniProt Code: | P51397 |
| NCBI GenInfo Identifier: | 1706298 |
| NCBI Gene ID: | 1611 |
| NCBI Accession: | P51397.3 |
| UniProt Secondary Accession: | P51397,Q6FGC3, Q9BUC9, |
| UniProt Related Accession: | P51397 |
| Molecular Weight: | 11,165 Da |
| NCBI Full Name: | Death-associated protein 1 |
| NCBI Synonym Full Names: | death associated protein |
| NCBI Official Symbol: | DAP�� |
| NCBI Protein Information: | death-associated protein 1 |
| UniProt Protein Name: | Death-associated protein 1 |
| Protein Family: | Dapper |
| UniProt Gene Name: | DAP�� |
| UniProt Entry Name: | DAP1_HUMAN |
