Description
| Product Name: | Human DAAO Recombinant Protein |
| Product Code: | RPPB1552 |
| Size: | 10µg |
| Species: | Human |
| Target: | DAAO |
| Synonyms: | D-amino-acid oxidase, DAMOX, DAAO, DAO, OXDA, MGC35381. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The DAAO solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MRVVVIGAGV IGLSTALCIH ERYHSVLQPL DIKVYADRFT PLTTTDVAAG LWQPYLSDPN NPQEADWSQQ TFDYLLSHVH SPNAENLGLF LISGYNLFHE AIPDPSWKDT VLGFRKLTPR ELDMFPDYGY GWFHTSLILE GKNYLQWLTE RLTERGVKFF QRKVESFEEV AREGADVIVN CTGVWAGALQ RDPLLQPGRG QIMKVDAPWM KHFILTHDPE RGIYNSPYII PGTQTVTLGG IFQLGNWSEL NNIQDHNTIW EGCCRLEPTL KNARIIGERT GFRPVRPQIR LEREQLRTGP SNTEVIHNYG HGGYGLTIHW GCALEAAKLF GRILEEKKLS RMPPSHL |
DAAO is a peroxisomal enzyme which uses FAD (flavin adenine dinucleotide) as a cofactor and oxidizes D-amino acids to the corresponding amino acids, producing ammonia and hydrogen peroxide. DAAO substrates include an extensive array of D-amino acids, but it is inactive on the naturally occurring L-amino acids. DAAO acts on a variety of D-amino acids especially on those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups; however it doesn�t act on acidic amino acids. DAAO may be involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino acids accumulated during aging. DAAO regulates the neuromodulator D-serine level in the brain. DAAO is highly active towards D-DOPA. Creatinine inhibits the DAAO in uremia. DAAO may also have a role in the pathophysiology of schizophrenia, but not in bipolar disorder.
DAAO Human Recombinant fused with a�20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 367 amino acids (1-347 a.a.) and having a molecular mass of 41.6kDa.The DAAO is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DAO: Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D- amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids. Belongs to the DAMOX/DASOX family. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - glycine, serine and threonine; Other Amino Acids Metabolism - D-Arginine and D-ornithine; Oxidoreductase; Amino Acid Metabolism - arginine and proline; EC 1.4.3.3 Chromosomal Location of Human Ortholog: 12q24 Cellular Component: peroxisomal membrane; peroxisomal matrix; mitochondrial outer membrane; peroxisome; cytosol Molecular Function:protein dimerization activity; protein binding; cofactor binding; D-amino-acid oxidase activity; receptor binding Biological Process: proline catabolic process; glyoxylate metabolic process; dopamine biosynthetic process; leucine metabolic process Disease: Schizophrenia |
| NCBI Summary: | This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P14920 |
| NCBI GenInfo Identifier: | 25453448 |
| NCBI Gene ID: | 1610 |
| NCBI Accession: | P14920.3 |
| UniProt Secondary Accession: | P14920,Q16758, Q8N6R2, B2R7I5, |
| UniProt Related Accession: | P14920 |
| Molecular Weight: | 39,474 Da |
| NCBI Full Name: | D-amino-acid oxidase |
| NCBI Synonym Full Names: | D-amino-acid oxidase |
| NCBI Official Symbol: | DAO�� |
| NCBI Official Synonym Symbols: | DAAO; OXDA; DAMOX�� |
| NCBI Protein Information: | D-amino-acid oxidase; D-amino acid oxidase |
| UniProt Protein Name: | D-amino-acid oxidase |
| Protein Family: | D-amino-acid oxidase |
| UniProt Gene Name: | DAO�� |
| UniProt Entry Name: | OXDA_HUMAN |
