Description
| Product Name: | Human Cyclophilin D Recombinant Protein |
| Product Code: | RPPB1539 |
| Size: | 20µg |
| Species: | Human |
| Target: | Cyclophilin D |
| Synonyms: | Peptidylprolyl isomerase D, PPID, CYPD, CYP-40, 40 kDa peptidyl-prolyl cis-trans isomerase, PPIase, Rotamase, Cyclophilin-40, CYP40, Cyclophilin-related protein, MGC33096, EC 5.2.1.8. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | 1mg/ml solution containing 1x PBS pH-7.4 & 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSHPSPQAKP SNPSNPRVFFDVDIGGERVG RIVLELFADI VPKTAENFRA LCTGEKGIGH TTGKPLHFKG CPFHRIIKKF MIQGGDFSNQ NGTGGESIYG EKFEDENFHY KHDREGLLSMANAGRNTNGS QFFITTVPTP HLDGKHVVFG QVIKGIGVAR ILENVEVKGEKPAKLCVIAE CGELKEGDDG GIFPKDGSGD SHPDFPEDAD IDLKDVDKIL LITEDLKNIG NTFFKSQNWE MAIKKYAEVL RYVDSSKAVI ETADRAKLQPIALSCVLNIG ACKLKMSNWQ GAIDSCLEAL ELDPSNTKAL YRRAQGWQGLKEYDQALADL KKAQGIAPED KAIQAELLKV KQKIKAQKDK EKAVYAKMFA |
| Biological Activity: | Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin. |
Cyclophilin-D is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and speeds up the protein folding. Cyclophilin-D possess PPIase activity and binds to the immunosuppressant cyclosporin-A. Cyclophilin-D is very well known that its overexpression suppresses the apoptosis in cancer cell. Cyclophilin-D suppresses apoptotic cell death by the use of mitochondrial hexokinase-2 dependent mechanism in cancer cells.
Cyclophilin-D Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (amino acids 1-370) containing 390 amino acids and having a molecular mass of 42.9kDa. Cyclophilin-D is fused to a 20 aa His Tag at N-terminus and is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PPID: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the cyclophilin-type PPIase family. PPIase D subfamily. |
| UniProt Protein Details: | Protein type:Nucleolus; Isomerase; EC 5.2.1.8 Chromosomal Location of Human Ortholog: 4q31.3 Cellular Component: cytoplasm; nucleolus; nucleus Molecular Function:cyclosporin A binding; estrogen receptor binding; heat shock protein binding; Hsp70 protein binding; Hsp90 protein binding; peptidyl-prolyl cis-trans isomerase activity; protein binding; transcription factor binding Biological Process: negative regulation of transcription from RNA polymerase II promoter; positive regulation of apoptosis; positive regulation of protein secretion; positive regulation of viral genome replication; protein complex assembly; protein folding; release of virus from host |
| NCBI Summary: | The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q08752 |
| NCBI GenInfo Identifier: | 729274 |
| NCBI Gene ID: | 5481 |
| NCBI Accession: | Q08752.3 |
| UniProt Secondary Accession: | Q08752,B2R9V2, |
| UniProt Related Accession: | Q08752 |
| Molecular Weight: | 40,764 Da |
| NCBI Full Name: | Peptidyl-prolyl cis-trans isomerase D |
| NCBI Synonym Full Names: | peptidylprolyl isomerase D |
| NCBI Official Symbol: | PPID�� |
| NCBI Official Synonym Symbols: | CYPD; CYP-40�� |
| NCBI Protein Information: | peptidyl-prolyl cis-trans isomerase D |
| UniProt Protein Name: | Peptidyl-prolyl cis-trans isomerase D |
| UniProt Synonym Protein Names: | 40 kDa peptidyl-prolyl cis-trans isomerase; Cyclophilin-40; CYP-40; Cyclophilin-related protein; Rotamase D |
| Protein Family: | Peptidyl-prolyl cis-trans isomerase |
| UniProt Gene Name: | PPID�� |
| UniProt Entry Name: | PPID_HUMAN |
