Description
| Product Name: | Human CS Recombinant Protein |
| Product Code: | RPPB1507 |
| Size: | 20µg |
| Species: | Human |
| Target: | CS |
| Synonyms: | Citrate Synthase, EC 2.3.3.1, Citrate (Si)-Synthase, EC2.3.3, Citrate synthase, mitochondrial. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | CS protein solution (1mg/ml) containing 20mM Tris-HClbuffer (pH 8.0), 0.15M NaCl,�10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSASSTNLK DILADLIPKE QARIKTFRQQ HGKTVVGQIT VDMMYGGMRGMKGLVYETSV LDPDEGIRFR GFSIPECQKL LPKAKGGEEP LPEGLFWLLV TGHIPTEEQV SWLSKEWAKRAALPSHVVTM LDNFPTNLHP MSQLSAAVTA LNSESNFARA YAQGISRTKY WELIYEDSMD LIAKLPCVAA KIYRNLYREG SGIGAIDSNLDWSHNFTNML GYTDHQFTEL TRLYLTIHSD HEGGNVSAHT SHLVGSALSD PYLSFAAAMN GLAGPLHGLANQEVLVWLTQ LQKEVGKDVS DEKLRDYIWN TLNSGRVVPG YGHAVLRKTD PRYTCQREFA LKHLPNDPMF KLVAQLYKIV PNVLLEQGKA KNPWPNVDAH SGVLLQYYGMTEMNYYTVLF GVSRALGVLA QLIWSRALGF PLERPKSMST EGLMKFVDSK SG |
Citrate synthase also known as CS is a Krebs tricarboxylic acid cycleenzyme which catalyzes the synthesis of citrate from oxaloacetate and acetylcoenzyme A. CS is present in almost all cells capable of oxidative metabolism.CS is nuclear encoded and transported into the mitochondrial matrix, where themature form is found. The diseases related to CS are: critical illnesspolyneuropathy and mitochondrial cardiomyopathy.
CS Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 462 amino acids (28-466 a.a) andhaving a molecular mass of 51.4kDa.CS is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CS: a mitochondrial enzyme of the tricarboxylic acid cycle that converts acetyl-CoA + H2O + oxaloacetate into citrate + CoA. Found in nearly all cells capable of oxidative metabolism. |
| UniProt Protein Details: | Protein type:EC 2.3.3.1; Mitochondrial; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Carbohydrate Metabolism - citrate (TCA) cycle; Transferase Chromosomal Location of Human Ortholog: 12q13.2 Cellular Component: mitochondrion; mitochondrial matrix; nucleus Molecular Function:citrate (Si)-synthase activity Biological Process: cellular metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; cellular carbohydrate metabolic process |
| NCBI Summary: | The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O75390 |
| NCBI GenInfo Identifier: | 57015285 |
| NCBI Gene ID: | 1431 |
| NCBI Accession: | O75390.2 |
| UniProt Secondary Accession: | O75390,Q71UT9, Q7KZH0, Q96FZ8, Q9BWN8, |
| UniProt Related Accession: | O75390 |
| Molecular Weight: | 466 |
| NCBI Full Name: | Citrate synthase, mitochondrial |
| NCBI Synonym Full Names: | citrate synthase |
| NCBI Official Symbol: | CS�� |
| NCBI Protein Information: | citrate synthase, mitochondrial; citrate (Si)-synthase |
| UniProt Protein Name: | Citrate synthase, mitochondrial |
| UniProt Synonym Protein Names: | Citrate (Si)-synthase |
| Protein Family: | Css54 |
| UniProt Gene Name: | CS�� |
| UniProt Entry Name: | CISY_HUMAN |
