Description
| Product Name: | Human CRYGS Recombinant Protein |
| Product Code: | RPPB3272 |
| Size: | 20µg |
| Species: | Human |
| Target: | CRYGS |
| Synonyms: | Crystallin gamma S, Gamma-crystallin S, CRYG8, crystallin, gamma 8, Beta-crystallin S. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The CRYGS solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMSKTGT KITFYEDKNF QGRRYDCDCD CADFHTYLSR CNSIKVEGGT WAVYERPNFA GYMYILPQGE YPEYQRWMGL NDRLSSCRAV HLPSGGQYKI QIFEKGDFSG QMYETTEDCP SIMEQFHMRE IHSCKVLEGV WIFYELPNYR GRQYLLDKKE YRKPIDWGAA SPAVQSFRRI VE |
Mammalian crystallins which are water soluble structural proteins located in the vertebrate eye are classified in three forms, labeled alpha, beta and gamma. Crystallins, the primary components of the lens, raise the refractive index of the eye all through the accommodation by creating high-molecular weight aggregates that maintain transparency. CRYGS is a monomer that does not aggregate. CRYGS encodes the most substantial gamma-crystallin in adult eye lens tissue. Gamma-crystallins has a part in cataract formation due to aging or mutations in specific genes,
CRYGS Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 202 amino acids (1-178) and having a molecular mass of 23.6 kDa.The CRYGS is fused to a 24 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CRYGS: Crystallins are the dominant structural components of the vertebrate eye lens. Belongs to the beta/gamma-crystallin family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 3q27.3 Disease: Cataract 20, Multiple Types |
| NCBI Summary: | Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. This gene encodes a protein initially considered to be a beta-crystallin but the encoded protein is monomeric and has greater sequence similarity to other gamma-crystallins. This gene encodes the most significant gamma-crystallin in adult eye lens tissue. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P22914 |
| NCBI GenInfo Identifier: | 4033688 |
| NCBI Gene ID: | 1427 |
| NCBI Accession: | P22914.4 |
| UniProt Secondary Accession: | P22914,B2RAF8, |
| UniProt Related Accession: | P22914 |
| Molecular Weight: | 21,007 Da |
| NCBI Full Name: | Beta-crystallin S |
| NCBI Synonym Full Names: | crystallin gamma S |
| NCBI Official Symbol: | CRYGS�� |
| NCBI Official Synonym Symbols: | CRYG8; CTRCT20�� |
| NCBI Protein Information: | beta-crystallin S |
| UniProt Protein Name: | Beta-crystallin S |
| UniProt Synonym Protein Names: | Gamma-S-crystallin; Gamma-crystallin S |
| Protein Family: | Beta-crystallin |
| UniProt Gene Name: | CRYGS�� |
| UniProt Entry Name: | CRBS_HUMAN |
