Description
| Product Name: | Human CRYGD Recombinant Protein |
| Product Code: | RPPB3269 |
| Size: | 20µg |
| Species: | Human |
| Target: | CRYGD |
| Synonyms: | Gamma-crystallin D, Gamma-D-crystallin, Gamma-crystallin 4, CRYGD, CRYG4, CCP; CACA, CCA3, cry-g-D. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | CRYGD protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 5mM DTT, 10% glycerol and 200mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH�MGKITLYEDR GFQGRHYECS SDHPNLQPYL SRCNSARVDS GCWMLYEQPN YSGLQYFLRR GDYADHQQWM GLSDSVRSCR LIPHSGSHRI RLYEREDYRG QMIEFTEDCS CLQDRFRFNE IHSLNVLEGS WVLYELSNYR GRQYLLMPGD YRRYQDWGAT NARVGSLRRV IDFS |
CRYGD is a member of the beta/gamma-crystallin family. Crystallins are the principal structural components of the vertebrate eye lens. The mammalian lens crystallins are divided into alpha, beta, and gamma families. Gamma-crystallins are involved in cataract formation. Defects in the CRYGD gene are responsible for cataract autosomal dominant (ADC), cataract congenital non-nuclear polymorphic autosomal dominant (CCP), cataract congenital cerulean type 3 (CCA3) and cataract crystalline aculeiform (CACA).
CRYGD Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 194 amino acids (1-174 a.a.) and having a molecular mass of 22.9kDa.CRYGD is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CRYGD: a major structural protein of the eye lens. A member of the beta/gamma-crystallin family. Defects are the cause of congenital zonular cataract with sutural opacities. Two splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 2q33.3 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding Biological Process: lens development in camera-type eye; visual perception Disease: Cataract 4, Multiple Types |
| NCBI Summary: | Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P07320 |
| NCBI GenInfo Identifier: | 2506321 |
| NCBI Gene ID: | 1421 |
| NCBI Accession: | P07320.3 |
| UniProt Secondary Accession: | P07320,Q17RF7, Q53R51, Q99681, |
| UniProt Related Accession: | P07320 |
| Molecular Weight: | 20,738 Da |
| NCBI Full Name: | Gamma-crystallin D |
| NCBI Synonym Full Names: | crystallin gamma D |
| NCBI Official Symbol: | CRYGD�� |
| NCBI Official Synonym Symbols: | CCP; PCC; CACA; CCA3; CRYG4; CTRCT4; cry-g-D�� |
| NCBI Protein Information: | gamma-crystallin D |
| UniProt Protein Name: | Gamma-crystallin D |
| UniProt Synonym Protein Names: | Gamma-D-crystallin; Gamma-crystallin 4 |
| Protein Family: | Gamma-crystallin |
| UniProt Gene Name: | CRYGD�� |
| UniProt Entry Name: | CRGD_HUMAN |
