Description
| Product Name: | Human CRMP1 Recombinant Protein |
| Product Code: | RPPB3259 |
| Size: | 20µg |
| Species: | Human |
| Target: | CRMP1 |
| Synonyms: | Dihydropyrimidinase-related protein 1, DRP-1, Collapsin response mediator protein 1, CRMP-1, CRMP1, DPYSL1, DRP1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | CRMP1 protein solution (1mg/ml) containing Phosphate buffered saline (pH7.4), 10% glycerol, 1mM DTT and 0.2mM PMSF. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSEFMSYQG KKSIPHITSD RLLIKGGRII NDDQSLYADV YLEDGLIKQI GENLIVPGGV KTIEANGRMV IPGGIDVNTY LQKPSQGMTA ADDFFQGTRA ALVGGTTMII DHVVPEPGSS LLTSFEKWHE AADTKSCCDY SLHVDITSWY DGVREELEVL VQDKGVNSFQ VYMAYKDVYQ MSDSQLYEAF TFLKGLGAVI LVHAENGDLI AQEQKRILEM GITGPEGHAL SRPEELEAEA VFRAITIAGR INCPVYITKV MSKSAADIIA LARKKGPLVF GEPIAASLGT DGTHYWSKNW AKAAAFVTSP PLSPDPTTPD YLTSLLACGD LQVTGSGHCP YSTAQKAVGK DNFTLIPEGV NGIEERMTVV WDKAVATGKM DENQFVAVTS TNAAKIFNLY PRKGRIAVGS DADVVIWDPD KLKTITAKSH KSAVEYNIFE GMECHGSPLV VISQGKIVFE DGNINVNKGM GRFIPRKAFP EHLYQRVKIR NKVFGLQGVS RGMYDGPVYE VPATPKYATP APSAKSSPSK HQPPPIRNLH QSNFSLSGAQ IDDNNPRRTG HRIVAPPGGR SNITSLG |
Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins involved in neuronal differentiation and axonal guidance. CRMP2 was previously shown to mediate the repulsive effect of Sema3A on axons and to participate in axonal specification. The X-ray crystal structure of murine CRMP1 was determined at 2.1 resolution and demonstrates that CRMP1 is a bilobed �lung-shaped� protein forming a tetrameric assembly.
CRMP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 597 amino acids (1-572 a.a) and having a molecular mass of 64.8kDa (Will appear as multiple bands on SDS-PAGE).CRMP1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CRMP-1: Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell development/differentiation; Cytoskeletal; Hydrolase Chromosomal Location of Human Ortholog: 4p16.1 Cellular Component: cell soma; cytoplasm; dendrite; microtubule organizing center; spindle; cytosol Molecular Function:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides; protein binding Biological Process: axon guidance; nervous system development; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; pyrimidine base catabolic process |
| NCBI Summary: | This gene encodes a member of a family of cytosolic phosphoproteins expressed exclusively in the nervous system. The encoded protein is thought to be a part of the semaphorin signal transduction pathway implicated in semaphorin-induced growth cone collapse during neural development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q14194 |
| NCBI GenInfo Identifier: | 3122031 |
| NCBI Gene ID: | 1400 |
| NCBI Accession: | Q14194.1 |
| UniProt Secondary Accession: | Q14194,Q13024, Q4W5F1, Q96TC8, A0EJG6, |
| UniProt Related Accession: | Q14194 |
| Molecular Weight: | 572 |
| NCBI Full Name: | Dihydropyrimidinase-related protein 1 |
| NCBI Synonym Full Names: | collapsin response mediator protein 1 |
| NCBI Official Symbol: | CRMP1�� |
| NCBI Official Synonym Symbols: | DRP1; DRP-1; CRMP-1; DPYSL1; ULIP-3�� |
| NCBI Protein Information: | dihydropyrimidinase-related protein 1; dihydropyrimidinase-like 1; unc-33-like phosphoprotein 3 |
| UniProt Protein Name: | Dihydropyrimidinase-related protein 1 |
| UniProt Synonym Protein Names: | Collapsin response mediator protein 1; CRMP-1; Unc-33-like phosphoprotein 3; ULIP-3 |
| Protein Family: | Dihydropyrimidinase-related protein |
| UniProt Gene Name: | CRMP1�� |
| UniProt Entry Name: | DPYL1_HUMAN |
