Description
| Product Name: | Human CPE Recombinant Protein |
| Product Code: | RPPB1469 |
| Size: | 20µg |
| Species: | Human |
| Target: | CPE |
| Synonyms: | Carboxypeptidase E, Carboxypeptidase H, CPH, CPE, CPE Human, Enkephalin convertase, Prohormone-processing carboxypeptidase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | CPE protein solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSLQQEDGI SFEYHRYPEL REALVSVWLQ CTAISRIYTV GRSFEGRELL VIELSDNPGV HEPGEPEFKY IGNMHGNEAV GRELLIFLAQ YLCNEYQKGN ETIVNLIHST RIHIMPSLNP DGFEKAASQP GELKDWFVGR SNAQGIDLNR NFPDLDRIVY VNEKEGGPNN HLLKNMKKIV DQNTKLAPET KAVIHWIMDI PFVLSANLHG GDLVANYPYD ETRSGSAHEY SSSPDDAIFQ SLARAYSSFN PAMSDPNRPP CRKNDDDSSF VDGTTNGGAW YSVPGGMQDF NYLSSNCFEI TVELSCEKFP PEETLKTYWE DNKNSLISYL EQIHRGVKGF VRDLQGNPIA NATISVEGID HDVTSAKDGD YWRLLIPGNY KLTASAPGYL AITKKVAVPY SPAAGVDFEL ESFSERKEEE KEELMEWWKM MSETLNF |
Carboxypeptidase-E (CPE ) is a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters. CPE is a peripheral membrane protein. CPE specifically connects regulated secretory pathway proteins, including prohormones, but constitutively secreted proteins. Mutations in CPE are implicated in type II diabetes.
CPE Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 457 amino acids (43-476 a.a.) and having a molecular mass of 51.4kDa. CPE is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CPE: Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protease; EC 3.4.17.10; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 4q32.3 Cellular Component: cell soma; extracellular space; Golgi apparatus; plasma membrane; secretory granule; secretory granule membrane Molecular Function:carboxypeptidase activity; cell adhesion molecule binding; metallocarboxypeptidase activity; neurexin binding; serine carboxypeptidase activity Biological Process: insulin processing; metabolic process; Wnt receptor signaling pathway |
| NCBI Summary: | This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes. [provided by RefSeq, Nov 2015] |
| UniProt Code: | P16870 |
| NCBI GenInfo Identifier: | 115892 |
| NCBI Gene ID: | 1363 |
| NCBI Accession: | P16870.1 |
| UniProt Secondary Accession: | P16870,Q9UIU9, A8K4N1, B3KR42, B4DFN4, D3DP33, |
| UniProt Related Accession: | P16870 |
| Molecular Weight: | 49,886 Da |
| NCBI Full Name: | Carboxypeptidase E |
| NCBI Synonym Full Names: | carboxypeptidase E |
| NCBI Official Symbol: | CPE�� |
| NCBI Official Synonym Symbols: | CPH�� |
| NCBI Protein Information: | carboxypeptidase E |
| UniProt Protein Name: | Carboxypeptidase E |
| UniProt Synonym Protein Names: | Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase |
| Protein Family: | Carboxypeptidase |
| UniProt Gene Name: | CPE�� |
| UniProt Entry Name: | CBPE_HUMAN |
