Description
| Product Name: | Human CLTB Recombinant Protein |
| Product Code: | RPPB3210 |
| Size: | 20µg |
| Species: | Human |
| Target: | CLTB |
| Synonyms: | Clathrin light chain B, Lcb, CLTB. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | CLTB protein solution (1mg/ml) containing 20mM Tris-HCl buffer, pH8.0, 10% glycerol, 0.2mM PMSF and 100mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMADDFGF FSSSESGAPE AAEEDPAAAF LAQQESEIAG IENDEGFGAP AGSHAAPAQP GPTSGAGSED MGTTVNGDVF QEANGPADGY AAIAQADRLT QEPESIRKWR EEQRKRLQEL DAASKVTEQE WREKAKKDLE EWNQRQSEQV EKNKINNRAS EEAFVKESKE ETPGTEWEKV AQLCDFNPKS SKQCKDVSRL RSVLMSLKQT PLSR |
Clathrin light chain B (CLTB) is a member of the clathrin light chain family. Clathrin is a protein that has a key role in the formation of coated vesicles. CLTB functions as the key structural component of the lattice-type cytoplasmic face of coated pits and vesicles which capture certain macromolecules during receptor-mediated endocytosis. CLTB forms a triskelion form comprised of 3 clathrin heavy chains and 3 light chains. When the triskelia interact they form a polyhedral pattern which encircles the vesicle. CME (Clathrin-mediated endocytosis) controls numerous cellular physiological processes such as the internalization of growth factors and receptors, entry of pathogens, and synaptic transmission.
CLTB Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 234 amino acids (1-211 a.a) and having a molecular mass of 25.6kDa (Molecular size on SDS-PAGE will appear higher).CLTB is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CLTB: Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts (via N-terminus) with HIP1. Interacts with HIP1R. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 5q35 Cellular Component: clathrin coat; clathrin coat of coated pit; clathrin coat of trans-Golgi network vesicle; clathrin vesicle coat; cytoplasm; intracellular membrane-bound organelle; plasma membrane; trans-Golgi network Molecular Function:clathrin heavy chain binding; peptide binding; protein binding; structural molecule activity Biological Process: intracellular protein transport |
| NCBI Summary: | Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P09497 |
| NCBI GenInfo Identifier: | 4502901 |
| NCBI Gene ID: | 1212 |
| NCBI Accession: | NP_001825.1 |
| UniProt Secondary Accession: | P09497,Q53Y37, Q6FHW1, |
| UniProt Related Accession: | P09497 |
| Molecular Weight: | 23,181 Da |
| NCBI Full Name: | clathrin light chain B isoform a |
| NCBI Synonym Full Names: | clathrin light chain B |
| NCBI Official Symbol: | CLTB�� |
| NCBI Official Synonym Symbols: | LCB�� |
| NCBI Protein Information: | clathrin light chain B |
| UniProt Protein Name: | Clathrin light chain B |
| Protein Family: | Clathrin |
| UniProt Gene Name: | CLTB�� |
| UniProt Entry Name: | CLCB_HUMAN |
