Description
| Product Name: | Human CLDN4 Recombinant Protein |
| Product Code: | RPPB3191 |
| Size: | 10µg |
| Species: | Human |
| Target: | CLDN4 |
| Synonyms: | Claudin 4, CPE-R, CPE-receptor, Clostridium perfringens enterotoxin receptor 1, WBSCR8, Williams-Beuren syndrome chromosomal region 8 protein, CPETR1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Filtered (0.4 �m) and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4. |
| Solubility: | It is recommended to add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10ug/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MKHHHHHHAS MWRVTAFIGS NIVTSQTIWE GLWMNCVVQS TGQMQCKVYD SLLALPQDLQ AAR LEVLFQG PTAHNIIQDF YNPLVASGQK REM MWRVTAFIGS NIVTSQTIWE GLWMNCVVQS TGQMQCKVYD SLLALPQDLQ AAR LEVLFQG PTAHNIIQDF YNPLVASGQK REM |
CLDN4 encodes an integral membrane protein that is a member of the claudin family. CLDN4 is a factor of tight junction strands and takes part in internal organ development and function in pre- and postnatal life. CLDN4 is removed in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems.
The CLDN4 Protein Human is produced in E. coli, and its molecular weight is 10.63 kDa. The protein containing 93 amino acid residues of the human CLDN4,10 amino acid N-Terminal HisTag residues and 8 additional amino acid PreScission protease cleavage site. The protein is a fusion protein consisting of two extracellular domains of claudin-4 linked together with the PreScission protease LEVLFQGP cleavage site.
| UniProt Protein Function: | Claudin-4: Plays a major role in tight junction-specific obliteration of the intercellular space. CLDN4 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Belongs to the claudin family. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: integral to plasma membrane; plasma membrane; tight junction Molecular Function:identical protein binding; transmembrane receptor activity Biological Process: calcium-independent cell-cell adhesion |
| NCBI Summary: | The protein encoded by this intronless gene belongs to the claudin family. Claudins are integral membrane proteins that are components of the epithelial cell tight junctions, which regulate movement of solutes and ions through the paracellular space. This protein is a high-affinity receptor for Clostridium perfringens enterotoxin (CPE) and may play a role in internal organ development and function during pre- and postnatal life. This gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems. [provided by RefSeq, Sep 2013] |
| UniProt Code: | O14493 |
| NCBI GenInfo Identifier: | 6685272 |
| NCBI Gene ID: | 1364 |
| NCBI Accession: | O14493.1 |
| UniProt Related Accession: | O14493 |
| Molecular Weight: | 22,077 Da |
| NCBI Full Name: | Claudin-4 |
| NCBI Synonym Full Names: | claudin 4 |
| NCBI Official Symbol: | CLDN4�� |
| NCBI Official Synonym Symbols: | CPER; CPE-R; CPETR; CPETR1; WBSCR8; hCPE-R�� |
| NCBI Protein Information: | claudin-4 |
| UniProt Protein Name: | Claudin-4 |
| UniProt Synonym Protein Names: | Clostridium perfringens enterotoxin receptor; CPE-R; CPE-receptor; Williams-Beuren syndrome chromosomal region 8 protein |
| Protein Family: | Claudin |
| UniProt Gene Name: | CLDN4�� |
| UniProt Entry Name: | CLD4_HUMAN |
