Description
| Product Name: | Human CENPU Recombinant Protein |
| Product Code: | RPPB3150 |
| Size: | 20µg |
| Species: | Human |
| Target: | CENPU |
| Synonyms: | Centromere Protein U, MLF1IP, Polo-Box-InteractingProtein 1, MLF1 Interacting Protein, Centromere Protein Of 50 KDa, InterphaseCentromere Complex Protein 24, KLIP1, PBIP1, KSHV Latent NuclearAntigen-Interacting Protein 1, MLF1-Interacting Protein, CENP50, CENPU50, KSHVLatent Nuclear Antigen Interacting Protein 1, CENP-50, CENP-U, ICEN24. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | CENPU protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol and0.4M Urea. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles. |
| Purity: | Greaterthan 80.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSTRRKVKS AEKISTQRHE VIRTTASSEL SEKPAESVTS KKTGPLSAQPSVEKENLAIE SQSKTQKKGK ISHDKRKKSR SKAIGSDTSD IVHIWCPEGM KTSDIKELNI VLPEFEKTHLEHQQRIESKV CKAAIATFYV NVKEQFIKML KESQMLTNLK RKNAKMISDI EKKRQRMIEV QDELLRLEPQ LKQLQTKYDE LKERKSSLRNAAYFLSNLKQ LYQDYSDVQA QEPNVKETYD SSSLPALLFK ARTLLGAESH LRNINHQLEK LLDQG |
Centromere Protein U, also known as CENPU is aspecialized chromatin domain, present throughout the cell cycle. CENPU performsas a platform on which the transient assembly of the kinetochore takes place duringmitosis. CENPU plays a major role in the correct PLK1 localization to themitotic kinetochores. A scaffold protein is responsible for the first recruitmentand maintenance of the kinetochore PLK1 population until its degradation. CENPUis also implicated in transcriptional repression.
CENPU Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 295 amino acids (147-418a.a) and having a molecular mass of 33.7kDa.CENPU is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CENPU: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Cell cycle regulationChromosomal Location of Human Ortholog: 4q35.1Cellular Component: cytosol; microtubule organizing center; nucleoplasm; nucleusMolecular Function: protein bindingBiological Process: DNA replication-independent nucleosome assembly at centromere; sister chromatid cohesion |
| UniProt Protein Details: | |
| NCBI Summary: | The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). MLF1IP, or CENPU, is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q71F23 |
| NCBI GenInfo Identifier: | 74712714 |
| NCBI Gene ID: | 79682 |
| NCBI Accession: | Q71F23.1 |
| UniProt Secondary Accession: | Q71F23,Q09GN2, Q32Q71, Q9H5G1, A2RRD9 |
| UniProt Related Accession: | Q71F23 |
| Molecular Weight: | 38,102 Da |
| NCBI Full Name: | Centromere protein U |
| NCBI Synonym Full Names: | centromere protein U |
| NCBI Official Symbol: | CENPU�� |
| NCBI Official Synonym Symbols: | KLIP1; PBIP1; CENP50; MLF1IP; CENPU50�� |
| NCBI Protein Information: | centromere protein U |
| UniProt Protein Name: | Centromere protein U |
| UniProt Synonym Protein Names: | Centromere protein of 50 kDa; CENP-50; Interphase centromere complex protein 24; KSHV latent nuclear antigen-interacting protein 1; MLF1-interacting protein; Polo-box-interacting protein 1 |
| Protein Family: | Centromere protein |
| UniProt Gene Name: | CENPU�� |
| UniProt Entry Name: | CENPU_HUMAN |
