Description
| Product Name: | Human CENPM Recombinant Protein |
| Product Code: | RPPB3147 |
| Size: | 10µg |
| Species: | Human |
| Target: | CENPM |
| Synonyms: | Centromere Protein M, Interphase Centromere Complex Protein 39, Chromosome 22 Open Reading Frame 18, Proliferation Associated Nuclear Element 1, bK250D10.2, CENP-M, ICEN39, C22orf18, PANE1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The CENPM solution contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 30% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMSVLRPL DKLPGLNTAT ILLVGTEDAL LQQLADSMLK EDCASELKVH LAKSLPLPSS VNRPRIDLIV FVVNLHSKYS LQNTEESLRH VDASFFLGKV CFLATGAGRE SHCSIHRHTV VKLAHTYQSP LLYCDLEVEG FRATMAQRLV RVLQICAGHV PGVSALNLLS LLRSSEGPSL EDL |
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays nucleosomes in which CENPA replaces histone H3. CENPM is an additional factor required for centromere assembly.
CENPM Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 203 amino acids (1-180) and having a molecular mass of 22.0kDa.CENPM is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CENPM: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. 3 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 22q13.2Cellular Component: nucleoplasm; cytosolBiological Process: nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; mitotic cell cycle |
| UniProt Protein Details: | |
| NCBI Summary: | The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9NSP4 |
| NCBI GenInfo Identifier: | 47117330 |
| NCBI Gene ID: | 79019 |
| NCBI Accession: | Q9NSP4.1 |
| UniProt Secondary Accession: | Q9NSP4,Q6I9W3, A7LM22, B1AHQ9 |
| UniProt Related Accession: | Q9NSP4 |
| Molecular Weight: | 11,687 Da |
| NCBI Full Name: | Centromere protein M |
| NCBI Synonym Full Names: | centromere protein M |
| NCBI Official Symbol: | CENPM�� |
| NCBI Official Synonym Symbols: | PANE1; CENP-M; C22orf18; bK250D10.2�� |
| NCBI Protein Information: | centromere protein M; interphase centromere complex protein 39; proliferation associated nuclear element 1; proliferation-associated nuclear element protein 1 |
| UniProt Protein Name: | Centromere protein M |
| UniProt Synonym Protein Names: | Interphase centromere complex protein 39; Proliferation-associated nuclear element protein 1 |
| Protein Family: | Centromere protein |
| UniProt Gene Name: | CENPM�� |
| UniProt Entry Name: | CENPM_HUMAN |
