Description
| Product Name: | Human CDC25A Recombinant Protein |
| Product Code: | RPPB5804 |
| Size: | 5µg |
| Species: | Human |
| Target: | CDC25A |
| Synonyms: | M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A, CDC25A2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The CDC25A solution (0.25 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 5mM DTT, 20% glycerol, 0.2M NaCl and 1mM EDTA. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 80.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMELG PEPPHRRRLL FACSPPPASQ PVVKALFGAS AAGGLSPVTN LTVTMDQLQG LGSDYEQPLE VKNNSNLQRM GSSESTDSGF CLDSPGPLDS KENLENPMRR IHSLPQKLLG CSPALKRSHS DSLDHDIFQL IDPDENKENE AFEFKKPVRP VSRGCLHSHG LQEGKDLFTQ RQNSAPARML SSNERDSSEP GNFIPLFTPQ SPVTATLSDE DDGFVDLLDG ENLKNEEETP SCMASLWTAP LVMRTTNLDN RCKLFDSPSL CSSSTRSVLK RPERSQEESP PGSTKRRKSM SGASPKESTN PEKAHETLHQ SLSLASSPKG TIENILDNDP RDLIGDFSKG YLFHTVAGKH QDLKYISPEI MASVLNGKFA NLIKEFVIID CRYPYEYEGG HIKGAVNLHM EEEVEDFLLK KPIVPTDGKR VIVVFHCEFS SERGPRMCRY VRERDRLGNE YPKLHYPELY VLKGGYKEFF MKCQSYCEPP SYRPMHHEDF KEDLKKFRTK SRTWAGEKSK REMYSRLKKL |
M-phase inducer phosphatase 1 (CDC25A) belongs to the CDC25 family of phosphatases. CDC25A is essential for progression from G1 to the S phase of the cell cycle. CDC25A activates the cyclin-dependent kinase CDC2 by eliminating 2 phosphate groups. CDC25A is specifically degraded in reaction to DNA damage, which inhibits cells with chromosomal abnormalities from progressing in the course of cell division. CDC25A is an oncogene, though its exact function in oncogenesis has not been determined.
CDC25A Human Recombinant fused with a�36 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing�560 amino acids (1-524 a.a.) and having a molecular mass of 63.2kDa. The CDC25A is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CDC25A: a member of the MPI phosphatase family. Functions as a dosage-dependent inducer in mitotic control. Required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two splice variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Protein phosphatase, dual-specificity; EC 3.1.3.48; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3p21 Cellular Component: nucleoplasm; cytosol Molecular Function:protein binding; protein tyrosine phosphatase activity; protein kinase binding Biological Process: cell proliferation; mitosis; response to radiation; cell division; regulation of cell cycle; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; DNA replication; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle |
| NCBI Summary: | CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P30304 |
| NCBI GenInfo Identifier: | 50403734 |
| NCBI Gene ID: | 993 |
| NCBI Accession: | P30304.2 |
| UniProt Secondary Accession: | P30304,Q8IZH5, Q96IL3, Q9H2F2, |
| UniProt Related Accession: | P30304 |
| Molecular Weight: | |
| NCBI Full Name: | M-phase inducer phosphatase 1 |
| NCBI Synonym Full Names: | cell division cycle 25A |
| NCBI Official Symbol: | CDC25A�� |
| NCBI Official Synonym Symbols: | CDC25A2�� |
| NCBI Protein Information: | M-phase inducer phosphatase 1; CDC25A2-CAG isoform; CDC25 isoform A1-CAG; dual specificity phosphatase CDC25A |
| UniProt Protein Name: | M-phase inducer phosphatase 1 |
| UniProt Synonym Protein Names: | Dual specificity phosphatase Cdc25A |
| Protein Family: | M-phase inducer phosphatase |
| UniProt Gene Name: | CDC25A�� |
| UniProt Entry Name: | MPIP1_HUMAN |
