Description
| Product Name: | Human CD320 Recombinant Protein |
| Product Code: | RPPB3060 |
| Size: | 10µg |
| Species: | Human |
| Target: | CD320 |
| Synonyms: | CD320 antigen isoform 1, CD320, 8D6, 8D6A, TCBLR, FDC-SM-8D6, FDC-signaling molecule 8D6. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | CD320 protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4), and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | LEAAASPLST PTSAQAAGPS SGSCPPTKFQ CRTSGLCVPL TWRCDRDLDC SDGSDEEECR IEPCTQKGQC PPPPGLPCPC TGVSDCSGGT DKKLRNCSRL ACLAGELRCT LSDDCIPLTW RCDGHPDCPD SSDELGCGTN EILPEGDATT MGPPVTLESV TSLRNATTMG PPVTLESVPS VGNATSSSAG DQSGSPTAYL EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH |
CD320 is a part of the low-density lipoprotein receptor family, having 2 low-density lipoprotein receptor type A domains divided by a complement-like cysteine-rich region. CD320 participates in cobalamin uptake�and internalizes the complex by endocytosis. CD320 is also plays a role as costimulator to promote B cell and follicular dendritic cells responses. CD320 is also known to augment the proliferation of PC precursors generated by IL-10.�
CD320 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 438 amino acids (31-229a.a.) and having a molecular mass of 47.4kDa. (Molecular size on SDS-PAGE will appear at approximately 57-70kDa). CD320 is expressed with a 239 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Receptor for transcobalamin saturated with cobalamin (TCbl) (PubMed:18779389). Plays an important role in cobalamin uptake (PubMed:18779389, PubMed:20524213). Plasma membrane protein that is expressed on follicular dendritic cells (FDC) and mediates interaction with germinal center B cells (PubMed:10727470). Functions as costimulator to promote B cell responses to antigenic stimuli; promotes B cell differentiation and proliferation (PubMed:10727470, PubMed:11418631). Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC) (PubMed:11418631). CD320 augments the proliferation of PC precursors generated by IL-10 (PubMed:11418631). |
| NCBI Summary: | This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin (vitamin B12), and is involved in B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011] |
| UniProt Code: | Q9NPF0 |
| NCBI GenInfo Identifier: | 74734303 |
| NCBI Gene ID: | 51293 |
| NCBI Accession: | Q9NPF0.1 |
| UniProt Secondary Accession: | Q9NPF0,Q53HF7, B2RDS5, D6W668, F5H6D3, |
| UniProt Related Accession: | Q9NPF0 |
| Molecular Weight: | 24,371 Da |
| NCBI Full Name: | CD320 antigen |
| NCBI Synonym Full Names: | CD320 molecule |
| NCBI Official Symbol: | CD320�� |
| NCBI Official Synonym Symbols: | 8D6; 8D6A; TCBLR�� |
| NCBI Protein Information: | CD320 antigen |
| UniProt Protein Name: | CD320 antigen |
| UniProt Synonym Protein Names: | 8D6 antigen |
| Protein Family: | CD320 antigen |
| UniProt Gene Name: | CD320�� |
