Description
| Product Name: | Human Carbohydrate Antigen 15-3 ELISA Kit |
| SKU: | HDES0107 |
| Size: | 96T |
| Target: | CA153 |
| Detection Method: | Sandwich ELISA |
| Result Type: | Quantitative: Sensitivity 98.7%, Specificity 99.5% |
| Sample Type: | Serum |
| Assay Time: | 1 h 15 min |
| Detection Wavelength: | 450/630nm |
| Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Indirect-ELISA as the principle to detect the Tuberculosis (TB) IgG in human serum, www.vetassay-elab.com 2 The content of CA15-3 in serum was detected by enzyme-linked immunosandwich method and avidin and biotin system. Firstly, avidin was coated with microporous plates to prepare solid aminotin, and then biotin-labeled antibodies and calibrators or serum to be tested were added. After incubation and washing, horseradish peroxidase-labeled MAB was added to form a complex of avidin-biotin-labeled MAB -[CA15-3-enzyme-labeled mab]. After color development, OD value was determined by enzyme-labeled MAB. The concentration - absorbance curve was fitted by computer or drawing, and the content of CA15-3 in serum was calculated inversely.
