Description
| Product Name: | Human C8G Recombinant Protein |
| Product Code: | RPPB2953 |
| Size: | 20µg |
| Species: | Human |
| Target: | C8G |
| Synonyms: | Complement component 8 gamma polypeptide, C8C, complement component C8 gamma chain, MGC142186. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The C8G solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 0.15M NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MQKPQRPRRP ASPISTIQPK ANFDAQQFAG TWLLVAVGSA CRFLQEQGHR AEATTLHVAP QGTAMAVSTF RKLDGICWQV RQLYGDTGVL GRFLLQARGA RGAVHVVVAE TDYQSFAVLY LERAGQLSVK LYARSLPVSD SVLSGFEQRV QEAHLTEDQI FYFPKYGFCE AADQFHVLDE VRR |
C8, a component of the complement system consists of three polypeptides C8A, C8B and C8G and takes part in the formation of Membrane Attack Complex (MAC). Patients with C8 deficiency are exposed to several bacteria infections. C8G, a member of the lipocalin family and calycin superfamily, is a secreted protein that can bind retinol. C8G is created in the liver, monocytes and fibroblast and has a role in clearing pathogens from an infected host.
C8G Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 203 amino acids (21-202) and having a molecular mass of 22.6 kDa.The C8G is fused to a 21 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | C8G: C8 is a constituent of the membrane attack complex. C8 binds to the C5B-7 complex, forming the C5B-8 complex. C5-B8 binds C9 and acts as a catalyst in the polymerization of C9. The gamma subunit seems to be able to bind retinol. Belongs to the calycin superfamily. Lipocalin family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 9q34.3 Cellular Component: membrane attack complex; extracellular region Molecular Function:complement binding; protein complex binding; retinol binding Biological Process: cytolysis; complement activation, alternative pathway; regulation of complement activation; innate immune response; complement activation, classical pathway |
| NCBI Summary: | The protein encoded by this gene belongs to the lipocalin family. It is one of the three subunits that constitutes complement component 8 (C8), which is composed of a disulfide-linked C8 alpha-gamma heterodimer and a non-covalently associated C8 beta chain. C8 participates in the formation of the membrane attack complex (MAC) on bacterial cell membranes. While subunits alpha and beta play a role in complement-mediated bacterial killing, the gamma subunit is not required for the bactericidal activity. [provided by RefSeq, Jul 2011] |
| UniProt Code: | P07360 |
| NCBI GenInfo Identifier: | 166197660 |
| NCBI Gene ID: | 733 |
| NCBI Accession: | NP_000597.2 |
| UniProt Secondary Accession: | P07360,Q14CT8, Q14CU0, Q5SQ07, |
| UniProt Related Accession: | P07360 |
| Molecular Weight: | 22,277 Da |
| NCBI Full Name: | complement component C8 gamma chain |
| NCBI Synonym Full Names: | complement component 8, gamma polypeptide |
| NCBI Official Symbol: | C8G�� |
| NCBI Official Synonym Symbols: | C8C�� |
| NCBI Protein Information: | complement component C8 gamma chain |
| UniProt Protein Name: | Complement component C8 gamma chain |
| Protein Family: | Complement component |
| UniProt Gene Name: | C8G�� |
| UniProt Entry Name: | CO8G_HUMAN |
