Description
| Product Name: | Human BUB3 Recombinant Protein |
| Product Code: | RPPB2917 |
| Size: | 20µg |
| Species: | Human |
| Target: | BUB3 |
| Synonyms: | BUB3L, hBUB3, Mitotic checkpoint protein BUB3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | BUB3 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMTGSNEF KLNQPPEDGI SSVKFSPNTS QFLLVSSWDT SVRLYDVPAN SMRLKYQHTG AVLDCAFYDP THAWSGGLDH QLKMHDLNTD QENLVGTHDA PIRCVEYCPE VNVMVTGSWD QTVKLWDPRT PCNAGTFSQP EKVYTLSVSG DRLIVGTAGR RVLVWDLRNM GYVQQRRESS LKYQTRCIRA FPNKQGYVLS SIEGRVAVEY LDPSPEVQKK KYAFKCHRLK ENNIEQIYPV NAISFHNIHN TFATGGSDGF VNIWDPFNKK RLCQFHRYPT SIASLAFSND GTTLAIASSY MYEMDDTEHP EDGIFIRQVT DAETKPKSPC T |
Mitotic checkpoint protein BUB3,( BUB3), is a saved component of the mitotic spindle assembly complex (MCC). The encoded protein has 4 WD repeat domains and has a similar sequence as the yeast BUB3 protein. BUB3 is vital for the kinetochore localization of BUB1 and BUBR1. BUB3 participates in the central spindle checkpoint pathway that operates during early embryogenesis. Furthermore BUB3 has a part in regulating the establishment of correct kinetochore-microtubule attachments.
BUB3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 351 amino acids (1-328 a.a.) and having a molecular mass of 39.5kDa.BUB3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | BUB3: Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Belongs to the WD repeat BUB3 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 10q26 Cellular Component: kinetochore; nucleoplasm; cytosol Molecular Function:protein binding Biological Process: negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic sister chromatid segregation; meiotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; attachment of spindle microtubules to kinetochore |
| NCBI Summary: | This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43684 |
| NCBI GenInfo Identifier: | 7387554 |
| NCBI Gene ID: | 9184 |
| NCBI Accession: | O43684.1 |
| UniProt Secondary Accession: | O43684,O43685, A6NJ42, B2R6E7, D3DRE9, |
| UniProt Related Accession: | O43684 |
| Molecular Weight: | 328 |
| NCBI Full Name: | Mitotic checkpoint protein BUB3 |
| NCBI Synonym Full Names: | BUB3 mitotic checkpoint protein |
| NCBI Official Symbol: | BUB3�� |
| NCBI Official Synonym Symbols: | BUB3L; hBUB3�� |
| NCBI Protein Information: | mitotic checkpoint protein BUB3; mitotic checkpoint component; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog; BUB3 budding uninhibited by benzimidazoles 3 homolog |
| UniProt Protein Name: | Mitotic checkpoint protein BUB3 |
| Protein Family: | BUB3-interacting and GLEBS motif-containing protein |
| UniProt Gene Name: | BUB3�� |
| UniProt Entry Name: | BUB3_HUMAN |
