Description
| Product Name: | Human BTC Recombinant Protein |
| Product Code: | RPPB0119 |
| Size: | 20µg |
| Species: | Human |
| Target: | BTC |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The Betacellulin Human Recombinant was lyophilized after extensive dialysis against 20mM phosphate buffer pH-7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized BTC Human in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Betacellulin Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BTC Human should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Gly-Asn-Ser-Thr |
| Biological Activity: | The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is < 0.05 ng/ml. corresponding to a Specific Activity of >20,000,000IU/mg. |
Btc is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. The effects of betacellulin are probably mediated by the egf receptor and other related receptors.
Betacellulin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 80 amino acids and having a molecular mass of 9 kDa. Betacellulin Human Recombinant is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | BTC: Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. |
| UniProt Protein Details: | Protein type:Ligand, receptor tyrosine kinase; Cell cycle regulation; Membrane protein, integral Chromosomal Location of Human Ortholog: 4q13.3 Cellular Component: extracellular space; plasma membrane; integral to membrane; extracellular region Molecular Function:protein binding; growth factor activity; epidermal growth factor receptor binding Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of fibroblast proliferation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of mitosis; positive regulation of cell proliferation; innate immune response; positive regulation of cell differentiation; negative regulation of apoptosis |
| NCBI Summary: | The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P35070 |
| NCBI GenInfo Identifier: | 461653 |
| NCBI Gene ID: | 685 |
| NCBI Accession: | P35070.1 |
| UniProt Secondary Accession: | P35070,Q96F48, |
| UniProt Related Accession: | P35070 |
| Molecular Weight: | 19,746 Da |
| NCBI Full Name: | Probetacellulin |
| NCBI Synonym Full Names: | betacellulin |
| NCBI Official Symbol: | BTC�� |
| NCBI Protein Information: | probetacellulin |
| UniProt Protein Name: | Probetacellulin |
| Protein Family: | Probetacellulin |
| UniProt Gene Name: | BTC�� |
| UniProt Entry Name: | BTC_HUMAN |
