Description
| Product Name: | Human BSG Recombinant Protein |
| Product Code: | RPPB2911 |
| Size: | 10µg |
| Species: | Human |
| Target: | BSG |
| Synonyms: | Basigin isoform 2, 5F7, CD147, EMMPRIN, OK, TCSF, Leukocyte activation antigen M6, OK blood group antigen. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | BSG protein solution (0.5mg/ml) contains phosphate buffered saline (pH7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPAAGTVFT TVEDLGSKIL LTCSLNDSAT EVTGHRWLKG GVVLKEDALP GQKTEFKVDS DDQWGEYSCV FLPEPMGTAN IQLHGPPRVK AVKSSEHINE GETAMLVCKS ESVPPVTDWA WYKITDSEDK ALMNGSESRF FVSSSQGRSE LHIENLNMEA DPGQYRCNGT SSKGSDQAIITLRVRSHLAL EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNGQPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH |
Basigin Isoform 2 or BSG is a part of the immunoglobulin superfamily, it is a type 1 transmembrane protein which has a variety of Nand O- glycosylation. BSG has several ligands, such as integrins, cyclophilin proteins Cyp-A & CyP-B. The protein has a crucial role in intercellular recognition, it takes part in many immunologic processes, differentiation and cell development. Also, it regulates numerus cell processes such as production and release of expression of the mono-carboxylate transporter, spermatogenesis, etc. Basigin Isoform 2 promotes invasion, metastasis, growth and survival of malignant cells by producing and releasing matrix metalloproteinases nearby mesenchymal cells and tumor cells.
BSG produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 428 amino acids (22-207a.a.) and having a molecular mass of 47.5kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).BSG is expressed with a 242 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | BSG: Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. Enriched on the surface of tumor cells. Up-regulated in gliomas. Its expression levels correlate with malignant potential of the tumor. Forms homooligomers in a cis-dependent manner on the plasma membrane. Forms a complex with MMP1 at the tumor cell surface. Interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. Interacts with ATP1B2, MAG and L1CAM. Interacts with AJAP1. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Immunoglobulin superfamily Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: acrosomal membrane; focal adhesion; Golgi membrane; integral to plasma membrane; lipid raft; melanosome; membrane; mitochondrion; plasma membrane; sarcolemma Molecular Function:mannose binding; protein binding Biological Process: blood coagulation; cell surface receptor linked signal transduction; cellular metabolic process; decidualization; embryo implantation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; leukocyte migration; odontogenesis of dentine-containing teeth; pyruvate metabolic process; response to cAMP; response to mercury ion; response to peptide hormone stimulus Disease: Blood Group--ok |
| NCBI Summary: | The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P35613 |
| NCBI GenInfo Identifier: | 51704273 |
| NCBI Gene ID: | 682 |
| NCBI Accession: | P35613.2 |
| UniProt Secondary Accession: | P35613,Q7Z796, Q8IZL7, A6NJW1, D3YLG5, |
| UniProt Related Accession: | P35613 |
| Molecular Weight: | 22,764 Da |
| NCBI Full Name: | Basigin |
| NCBI Synonym Full Names: | basigin (Ok blood group) |
| NCBI Official Symbol: | BSG�� |
| NCBI Official Synonym Symbols: | OK; 5F7; TCSF; CD147; EMMPRIN�� |
| NCBI Protein Information: | basigin |
| UniProt Protein Name: | Basigin |
| UniProt Synonym Protein Names: | 5F7; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; EMMPRIN; Leukocyte activation antigen M6; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor; TCSF; CD_antigen: CD147 |
| Protein Family: | Basigin |
| UniProt Gene Name: | BSG�� |
| UniProt Entry Name: | BASI_HUMAN |
