Description
| Product Name: | Human BCDIN3D Recombinant Protein |
| Product Code: | RPPB2860 |
| Size: | 10µg |
| Species: | Human |
| Target: | BCDIN3D |
| Synonyms: | Pre-miRNA 5'-monophosphate methyltransferase, BCDIN3 domain-containing protein, BCDIN3D. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | BCDIN3D protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol, 1mM DTT and 2mM EDTA. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAVPTEL DGGSVKETAA EEESRVLAPG AAPFGNFPHY SRFHPPEQRL RLLPPELLRQ LFPESPENGP ILGLDVGCNS GDLSVALYKH FLSLPDGETC SDASREFRLL CCDIDPVLVK RAEKECPFPD ALTFITLDFM NQRTRKVLLS SFLSQFGRSV FDIGFCMSIT MWIHLNHGDH GLWEFLAHLS SLCHYLLVEP QPWKCYRAAA RRLRKLGLHD FDHFHSLAIR GDMPNQIVQI LTQDHGMELI CCFGNTSWDR SLLLFRAKQT IETHPIPESL IEKGKEKNRL SFQKQ |
BCDIN3D is a member of the methyltransferase superfamily and contains 1 Bin3-type SAM domain. BCDIN3D acts in the catalysis of the transfer of a methyl group to an acceptor molecule. BCDIN3D is an O-methyltransferase which specifically dimethylates the 5' monophosphate of pre-miRNAs, serving as a negative regulator of miRNA processing. BCDIN3D mediates the methylation of pre-miR-145, as well as other pre-miRNAs.
BCDIN3D Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 315 amino acids (1-292 a.a) and having a molecular mass of 35kDa.BCDIN3D is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | BCDIN3D: Probable methyltransferase. Belongs to the methyltransferase superfamily. |
| UniProt Protein Details: | Protein type:EC 2.1.1.-; Methyltransferase Chromosomal Location of Human Ortholog: 12q13.12 Cellular Component: cytoplasm; nucleus Molecular Function:O-methyltransferase activity; protein binding; RNA methyltransferase activity Biological Process: RNA methylation |
| UniProt Code: | Q7Z5W3 |
| NCBI GenInfo Identifier: | 74738762 |
| NCBI Gene ID: | 144233 |
| NCBI Accession: | Q7Z5W3.1 |
| UniProt Secondary Accession: | Q7Z5W3,A8K829, |
| UniProt Related Accession: | Q7Z5W3 |
| Molecular Weight: | 33,200 Da |
| NCBI Full Name: | Pre-miRNA 5'-monophosphate methyltransferase |
| NCBI Synonym Full Names: | BCDIN3 domain containing RNA methyltransferase |
| NCBI Official Symbol: | BCDIN3D�� |
| NCBI Protein Information: | pre-miRNA 5'-monophosphate methyltransferase |
| UniProt Protein Name: | Pre-miRNA 5'-monophosphate methyltransferase |
| UniProt Synonym Protein Names: | BCDIN3 domain-containing protein |
| Protein Family: | Pre-miRNA 5'-monophosphate methyltransferase |
| UniProt Gene Name: | BCDIN3D�� |
| UniProt Entry Name: | BN3D2_HUMAN |
