Description
| Product Name: | Human BCCIP Recombinant Protein |
| Product Code: | RPPB2859 |
| Size: | 10µg |
| Species: | Human |
| Target: | BCCIP |
| Synonyms: | BRCA2 and CDKN1A-interacting protein, P21- and CDK-associated protein 1, Protein TOK-1, BCCIP, TOK1, BRCA2 and CDKN1A-interacting protein. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | BCCIP protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSEFMASRS KRRAVESGVP QPPDPPVQRD EEEEKEVENE DEDDDDSDKE KDEEDEVIDE EVNIEFEAYS LSDNDYDGIK KLLQQLFLKA PVNTAELTDL LIQQNHIGSV IKQTDVSEDS NDDMDEDEVF GFISLLNLTE RKGTQCVEQI QELVLRFCEK NCEKSMVEQL DKFLNDTTKP VGLLLSERFI NVPPQIALPM YQQLQKELAG AHRTNKPCGK CYFYLLISKT FVEAGKNNSK KKPSNKKKAA LMFANAEEEF FYEKAILKFN YSVQEESDTC LGGKWSFDDV PMTPLRTVML IPGDKMNEIM DKLKEYLSV |
BRCA2 And CDKN1A Interacting Protein (BCCIP) is a nuclear protein with multiple interacting domains which remained conserved throughout the evolution. BCCIP is an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. BCCIP takes also part in the regulation of BRCA2 and RAD51 nuclear focus formation, doublestrand break-induced homologous recombination, and cell cycle progression.
BCCIP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 339 amino acids (1-314 a.a.) and having a molecular mass of 38.6kDa.BCCIP is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | BCCIP: May promote cell cycle arrest by enhancing the inhibition of CDK2 activity by CDKN1A. May be required for repair of DNA damage by homologous recombination in conjunction with BRCA2. May not be involved in non-homologous end joining (NHEJ). Belongs to the BCP1 family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 10q26.1 Cellular Component: nuclear cyclin-dependent protein kinase holoenzyme complex; nucleus Molecular Function:protein binding; kinase regulator activity Biological Process: regulation of cyclin-dependent protein kinase activity; cell cycle; DNA repair |
| NCBI Summary: | This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9P287 |
| NCBI GenInfo Identifier: | 7706581 |
| NCBI Gene ID: | 56647 |
| NCBI Accession: | NP_057651.1 |
| UniProt Secondary Accession: | Q9P287,Q8ND15, Q96GC4, Q9P288, B3KP45, |
| UniProt Related Accession: | Q9P287 |
| Molecular Weight: | 33,242 Da |
| NCBI Full Name: | BRCA2 and CDKN1A-interacting protein isoform BCCIPalpha |
| NCBI Synonym Full Names: | BRCA2 and CDKN1A interacting protein |
| NCBI Official Symbol: | BCCIP�� |
| NCBI Official Synonym Symbols: | TOK1; TOK-1�� |
| NCBI Protein Information: | BRCA2 and CDKN1A-interacting protein; BCCIPbeta; TOK-1beta; BCCIPalpha; TOK-1alpha; protein TOK-1; cdk inhibitor p21 binding protein; p21- and CDK-associated protein 1; BRCA2 and Cip1/p21 interacting protein |
| UniProt Protein Name: | BRCA2 and CDKN1A-interacting protein |
| UniProt Synonym Protein Names: | P21- and CDK-associated protein 1; Protein TOK-1 |
| Protein Family: | BRCA2 and CDKN1A-interacting protein |
| UniProt Gene Name: | BCCIP�� |
| UniProt Entry Name: | BCCIP_HUMAN |
