Description
| Product Name: | Human BCAS2 Recombinant Protein |
| Product Code: | RPPB2858 |
| Size: | 5µg |
| Species: | Human |
| Target: | BCAS2 |
| Synonyms: | Pre-mRNA-splicing factor SPF27, Breast carcinoma-amplified sequence 2, DNA amplified in mammary carcinoma 1 protein, Spliceosome-associated protein SPF 27, BCAS2, DAM1, SPF27, Snt309. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The BCAS2 solution (0.25 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 5mM DTT and 0.2M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAGT GLVAGEVVVD ALPYFDQGYE APGVREAAAA LVEEETRRYR PTKNYLSYLT APDYSAFETD IMRNEFERLA ARQPIELLSM KRYELPAPSS GQKNDITAWQ ECVNNSMAQL EHQAVRIENL ELMSQHGCNA WKVYNENLVH MIEHAQKELQ KLRKHIQDLN WQRKNMQLTA GSKLREMESN WVSLVSKNYE IERTIVQLEN EIYQIKQQHG EANKENIRQD F |
Pre-mRNA-splicing factor SPF27 (BCAS2) is a ubiquitously expressed nuclear protein that was initially identified as being overexpressed in various breast cancer cell lines. BCAS2 is currently as a component of the spliceosome, participating in the exclusion of introns from mRNA precursors. BCAS2 acts as an ER co-activator and is capable of boosting ER-mediated transcription, suggesting that BCAS2 is involved in the development of breast cancer.
BCAS2 Human Recombinant fused with a�36 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing�261 amino acids (1-225 a.a.) and having a molecular mass of 30.3kDa. The BCAS2 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | BCAS2: Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Belongs to the SPF27 family. |
| UniProt Protein Details: | Protein type:Nucleolus; RNA splicing; Spliceosome; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 1p13.2 Cellular Component: nucleoplasm; spliceosome; DNA replication factor A complex; nucleolus; nucleus; cell junction Molecular Function:protein binding Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing, via transesterification reactions; RNA splicing; gene expression |
| UniProt Code: | O75934 |
| NCBI GenInfo Identifier: | 71153839 |
| NCBI Gene ID: | 10286 |
| NCBI Accession: | O75934.1 |
| UniProt Secondary Accession: | O75934,Q6FGS0, |
| UniProt Related Accession: | O75934 |
| Molecular Weight: | 225 |
| NCBI Full Name: | Pre-mRNA-splicing factor SPF27 |
| NCBI Synonym Full Names: | breast carcinoma amplified sequence 2 |
| NCBI Official Symbol: | BCAS2�� |
| NCBI Official Synonym Symbols: | DAM1; SPF27; Snt309�� |
| NCBI Protein Information: | pre-mRNA-splicing factor SPF27; breast carcinoma-amplified sequence 2; spliceosome-associated protein SPF 27; DNA amplified in mammary carcinoma 1 protein; spliceosome associated protein, amplified in breast cancer |
| UniProt Protein Name: | Pre-mRNA-splicing factor SPF27 |
| UniProt Synonym Protein Names: | Breast carcinoma-amplified sequence 2; DNA amplified in mammary carcinoma 1 protein; Spliceosome-associated protein SPF 27 |
| UniProt Gene Name: | BCAS2�� |
| UniProt Entry Name: | SPF27_HUMAN |
