Description
| Product Name: | Human BCAR1 Recombinant Protein |
| Product Code: | RPPB2857 |
| Size: | 10µg |
| Species: | Human |
| Target: | BCAR1 |
| Synonyms: | CAS, CAS1, CASS1, CRKAS, P130Cas, Breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, Cas scaffolding protein family member 1, BCAR1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The BCAR1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M UREA and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSRLQQGVS ATVAHLLDLA GSAGATGSWR SPSEPQEPLV QDLQAAVAAV QSAVHELLEF ARSAVGNAAH TSDRALHAKL SRQLQKMEDV HQTLVAHGQA LDAGRGGSGA TLEDLDRLVA CSRAVPEDAK QLASFLHGNA SLLFRRTKAT APGPEGGGTL HPNPTDKTSS IQSRPLPSPP KFTSQDSPDG QYENSEGGWM EDYDYVHLQG KEEFEKTQKE LLEKGSITRQ GKSQLELQQL KQFERLEQEV SRPIDHDLAN WTPAQPLAPG RTGGLGPSDR QLLLFYLEQC EANLTTLTNA VDAFFTAVAT NQPPKIFVAH SKFVILSAHK LVFIGDTLSR QAKAADVRSQ VTHYSNLLCD LLRGIVATTK AAALQYPSPS AAQDMVE |
Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) is a Src family kinase substrate which takes part in a range of cellular events such as migration, survival, transformation, and invasion. BCAR1 plays a vital role for tyrosine kinase-based signaling related to cell adhesion.
BCAR1 Human Recombinant produced in E. coli is. a single polypeptide chain containing 407 amino acids (465-848) and having a molecular mass of 43.9kDa. BCAR1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | P130Cas: a docking protein containing multiple protein-protein interaction domains. Plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. The amino-terminal SH3 domain regulates its interaction with focal adhesion kinase (FAK) and the FAK-related kinase PYK2 and also with tyrosine phosphatases PTP-1B and PTP-PEST. Overexpression confers antiestrogen resistance on breast cancer cells. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold Chromosomal Location of Human Ortholog: 16q23.1 Cellular Component: ruffle; focal adhesion; lamellipodium; cytoplasm; nucleolus; plasma membrane; cytosol; actin cytoskeleton Molecular Function:signal transducer activity; protein binding; SH3 domain binding; protein kinase binding Biological Process: epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; antigen receptor-mediated signaling pathway; platelet activation; cell migration; nerve growth factor receptor signaling pathway; actin filament organization; hepatocyte growth factor receptor signaling pathway; platelet-derived growth factor receptor signaling pathway; T cell receptor signaling pathway; regulation of apoptosis; G-protein coupled receptor protein signaling pathway; cell proliferation; B cell receptor signaling pathway; cell division; insulin receptor signaling pathway; regulation of cell growth; cell adhesion; vascular endothelial growth factor receptor signaling pathway; blood coagulation; positive regulation of cell migration |
| NCBI Summary: | BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009] |
| UniProt Code: | P56945 |
| NCBI GenInfo Identifier: | 288558806 |
| NCBI Gene ID: | 9564 |
| NCBI Accession: | P56945.2 |
| UniProt Secondary Accession: | P56945,B3KWD7, B4DEV4, B4DGB5, B4DIW5, B7Z7X7, E9PCL5 E9PCV2, F5GXA2, F5GXV6, F5H7Z0, F8WA69, |
| UniProt Related Accession: | P56945 |
| Molecular Weight: | 870 |
| NCBI Full Name: | Breast cancer anti-estrogen resistance protein 1 |
| NCBI Synonym Full Names: | breast cancer anti-estrogen resistance 1 |
| NCBI Official Symbol: | BCAR1�� |
| NCBI Official Synonym Symbols: | CAS; CAS1; CASS1; CRKAS; P130Cas�� |
| NCBI Protein Information: | breast cancer anti-estrogen resistance protein 1; Crk-associated substrate p130Cas; Cas scaffolding protein family member 1 |
| UniProt Protein Name: | Breast cancer anti-estrogen resistance protein 1 |
| UniProt Synonym Protein Names: | CRK-associated substrate; Cas scaffolding protein family member 1; p130cas |
| Protein Family: | Breast cancer anti-estrogen resistance protein |
| UniProt Gene Name: | BCAR1�� |
| UniProt Entry Name: | BCAR1_HUMAN |
