The Human Autophagy Array - 20 Targets (SARB0023) is a powerful tool for researchers studying the process of autophagy, a critical cellular mechanism involved in maintaining cellular homeostasis and responding to stress. This array allows for the simultaneous detection and quantification of 20 key autophagy-related proteins, providing valuable insights into the regulation of this important cellular process.With a focus on key autophagy targets such as LC3, p62, Beclin-1, and ATG5, this array enables researchers to study the dynamic changes in protein expression levels associated with autophagy induction and progression. The array is designed for use with human samples, offering researchers a comprehensive overview of autophagy-related protein expression in various cell types and biological contexts.
Whether investigating the role of autophagy in cancer, neurodegenerative diseases, or metabolic disorders, the Human Autophagy Array - 20 Targets (SARB0023) provides a reliable and efficient platform for analyzing multiple autophagy-related proteins in a single experiment. By gaining a deeper understanding of the molecular mechanisms governing autophagy, researchers can identify novel therapeutic targets and develop potential treatments for a wide range of diseases.
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
ATG12
Gene Symbol: ATG12 APG12 APG12L
Gene ID: 9140
Accession #: O94817
ATG7
Gene Symbol: ATG7 APG7L
Gene ID: 10533
Accession #: O95352
ATG10
Gene Symbol: ATG10 APG10L PP12616
Gene ID: 83734
Accession #: Q9H0Y0
ATG13
Gene Symbol: ATG13 KIAA0652
Gene ID: 9776
Accession #: O75143
ATG3
Gene Symbol: ATG3 APG3 APG3L
Gene ID: 64422
Accession #: Q9NT62
ATG4A
Gene Symbol: ATG4A APG4A AUTL2
Gene ID: 115201
Accession #: Q8WYN0
ATG4B
Gene Symbol: ATG4B APG4B AUTL1 KIAA0943
Gene ID: 23192
Accession #: Q9Y4P1
ATG5
Gene Symbol: ATG5 APG5L ASP
Gene ID: 9474
Accession #: Q9H1Y0
Beclin
Gene Symbol: BECN1 GT197
Gene ID: 8678
Accession #: Q14457
BNIP3L
Gene Symbol: BNIP3L BNIP3A BNIP3H NIX
Gene ID: 665
Accession #: O60238
DDR2
Gene Symbol: DDR2 NTRKR3 TKT TYRO10
Gene ID: 4921
Accession #: Q16832
GABARAP
Gene Symbol: GABARAP FLC3B HT004
Gene ID: 11337
Accession #: O95166
LAMP1 (CD107a)
Gene Symbol: LAMP1
Gene ID: 3916
Accession #: P11279
LC3a
Gene Symbol: MAP1LC3A
Gene ID: 84557
Accession #: Q9H492
LC3B
Gene Symbol: MAP1LC3B MAP1ALC3
Gene ID: 81631
Accession #: Q9GZQ8
NBS1
Gene Symbol: NBN NBS NBS1 P95
Gene ID: 4683
Accession #: O60934
P62
Gene Symbol: SQSTM1 ORCA OSIL
Gene ID: 8878
Accession #: Q13501
RHEB
Gene Symbol: RHEB RHEB2
Gene ID: 6009
Accession #: Q15382
MSK1
Gene Symbol: RPS6KA5 MSK1
Gene ID: 9252
Accession #: O75582
Alpha-synuclein
Gene Symbol: SNCA NACP PARK1
Gene ID: 6622
Accession #: P37840
Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex
Human Autophagy Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Other Materials Required
Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.
Block membranes
2.
Incubate with Sample
3.
Incubate with Biotinylated Detection Antibody Cocktail
4.
Incubate with HRP-Conjugated Streptavidin
5.
Incubate with Detection Buffers
6.
Image with chemiluminescent imaging system
7.
Perform densitometry and analysis
Ágnes Tósaki et al.
A new cannabigerol derivative, LE-127/2, induces autophagy mediated cell death in human cutaneous melanoma cells
