Description
| Product Name: | Human ATP6V1F Recombinant Protein |
| Product Code: | RPPB2827 |
| Size: | 20µg |
| Species: | Human |
| Target: | ATP6V1F |
| Synonyms: | ATP6S14, VATF, Vma7, V-type proton ATPase subunit F, V-ATPase14 kDa subunit. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | ATP6V1F protein solution (0.5mg/ml) containing Phosphate buffered saline (pH7.4), 50% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAGRGKL IAVIGDEDTV TGFLLGGIGE LNKNRHPNFL VVEKDTTINEIEDTFRQFLN RDDIGIILIN QYIAEMVRHA LDAHQQSIPA VLEIPSKEHP YDAAKDSILR RARGMFTAED LR |
ATPase Transporting, Lysosomal V1 Subunit F (ATP6V1F) is a component of vacuolar ATPase (V-ATPase); it is a multi-subunit enzyme, which mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is required for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain contains 3 A and 3 B subunits, 2 G subunits as well as the C, D, E, F, and H subunits. The V1 domain has the ATP catalytic site. The V0 domain consists of 5 different subunits: a, c, c', c', and d.
ATP6V1F Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 142 amino acids (1-119 a.a) and having a molecular mass of 15.8kDa.ATP6V1F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ATP6V1F: Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase F subunit family.Protein type: HydrolaseChromosomal Location of Human Ortholog: 7q32Cellular Component: cytosol; membrane; vacuolar proton-transporting V-type ATPase complexMolecular Function: ATPase activity, coupled to transmembrane movement of ions; protein bindingBiological Process: insulin receptor signaling pathway; transferrin transport |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q16864 |
| NCBI GenInfo Identifier: | 126302612 |
| NCBI Gene ID: | 9296 |
| NCBI Accession: | Q16864.2 |
| UniProt Secondary Accession: | Q16864,Q6IBA8, C9J2K4 |
| UniProt Related Accession: | Q16864 |
| Molecular Weight: | 16,402 Da |
| NCBI Full Name: | V-type proton ATPase subunit F |
| NCBI Synonym Full Names: | ATPase H+ transporting V1 subunit F |
| NCBI Official Symbol: | ATP6V1F�� |
| NCBI Official Synonym Symbols: | VATF; Vma7; ATP6S14�� |
| NCBI Protein Information: | V-type proton ATPase subunit F |
| UniProt Protein Name: | V-type proton ATPase subunit F |
| UniProt Synonym Protein Names: | V-ATPase 14 kDa subunit; Vacuolar proton pump subunit F |
| Protein Family: | |
| UniProt Gene Name: | ATP6V1F�� |
| UniProt Entry Name: | VATF_HUMAN |
