Description
| Product Name: | Human ATP5H Recombinant Protein |
| Product Code: | RPPB2824 |
| Size: | 10µg |
| Species: | Human |
| Target: | ATP5H |
| Synonyms: | ATP Synthase H+ Transporting, Mitochondrial Fo Complex Subunit D, ATP Synthase D Chain Mitochondrial, ATP Synthase H+ Transporting Mitochondrial F1F0 Subunit D, ATPase Subunit D, My032 Protein, ATPQ. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The ATP5H solution contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAGRKLA LKTIDWVAFA EIIPQNQKAI ASSLKSWNET LTSRLAALPE NPPAIDWAYY KANVAKAGLV DDFEKKFNAL KVPVPEDKYT AQVDAEEKED VKSCAEWVSL SKARIVEYEK EMEKMKNLIP FDQMTIEDLN EAFPETKLDK KKYPYWPHQP IENL |
ATP5H is a member of the ATPase d subunit family and encodes the d subunit of the F0 complex. Electron transport complexes of the respiratory chain create a proton gradient across the membrane which induces ATP5H to transform ADP to ATP. Two alternatively spliced isoforms of ATP5H, encoded by a gene that maps to human chromosome 17q25.1, are known. ATP5H is restricted to mitochondrial inner membrane.
ATP5H Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 184 amino acids (1-161) and having a molecular mass of 20.9kDa.ATP5H is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ATP5H: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Belongs to the ATPase d subunit family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial; Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14 Chromosomal Location of Human Ortholog: 17q25 Cellular Component: nucleoplasm; mitochondrion; cytoplasm; mitochondrial inner membrane; mitochondrial proton-transporting ATP synthase complex Molecular Function:ATPase activity; transmembrane transporter activity; hydrogen ion transmembrane transporter activity Biological Process: cellular metabolic process; mitochondrial ATP synthesis coupled proton transport |
| NCBI Summary: | Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq, Jun 2010] |
| UniProt Code: | O75947 |
| NCBI GenInfo Identifier: | 6831494 |
| NCBI Gene ID: | 10476 |
| NCBI Accession: | O75947.3 |
| UniProt Secondary Accession: | O75947,Q9H3J4, B2R5L6, |
| UniProt Related Accession: | O75947 |
| Molecular Weight: | Observed MW 18kDaCalculated MW 15kDa/18kDa |
| NCBI Full Name: | ATP synthase subunit d, mitochondrial |
| NCBI Synonym Full Names: | ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d |
| NCBI Official Symbol: | ATP5H�� |
| NCBI Official Synonym Symbols: | ATPQ�� |
| NCBI Protein Information: | ATP synthase subunit d, mitochondrial; My032 protein; ATPase subunit d; ATP synthase D chain, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1F0, subunit d; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d |
| UniProt Protein Name: | ATP synthase subunit d, mitochondrial |
| UniProt Gene Name: | ATP5H�� |
| UniProt Entry Name: | ATP5H_HUMAN |
