Description
| Product Name: | Human ATP1B1 Recombinant Protein |
| Product Code: | RPPB2818 |
| Size: | 20µg |
| Species: | Human |
| Target: | ATP1B1 |
| Synonyms: | Sodium/potassium-transporting ATPase subunit beta-1, ATPase, Na+/K+ transporting, beta 1 polypeptide, ATP1B, ATPBS, Sodium/potassium-dependent ATPase subunit beta-1, ATP1B1, ATPaseTransporting Beta 1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The ATP1B1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSEFKPTYQ DRVAPPGLTQ IPQIQKTEIS FRPNDPKSYE AYVLNIVRFL EKYKDSAQRD DMIFEDCGDV PSEPKERGDF NHERGERKVC RFKLEWLGNC SGLNDETYGY KEGKPCIIIK LNRVLGFKPK PPKNESLETY PVMKYNPNVL PVQCTGKRDE DKDKVGNVEY FGLGNSPGFP LQYYPYYGKL LQPKYLQPLL AVQFTNLTMD TEIRIECKAY GENIGYSEKD RFQGRFDVKI EVKS |
ATPaseTransporting Beta 1 (ATP1B1) is a part of the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an essential membrane protein accountable for establishing and maintaining the electrochemical gradients of Na and K ions over the plasma membrane. These gradients are vital for osmoregulation, for sodium-coupled transport of a range of organic and inorganic molecules, and for electrical excitability of muscle and nerve. ATP1B1is combined of 2 subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates the number of sodium pumps transported to the plasma membrane through assembly of alpha/beta heterodimers. ATP1B1 is a beta 1 subunit.
ATP1B1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 264 amino acids (63-303) and having a molecular mass of 30.4 kDa.ATP1B1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ATP1B1: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Belongs to the X(+)/potassium ATPases subunit beta family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transporter; Membrane protein, integral Chromosomal Location of Human Ortholog: 1q24 Cellular Component: apical plasma membrane; basolateral plasma membrane; caveola; intracellular; membrane; myelin sheath; plasma membrane; sarcolemma; sodium:potassium-exchanging ATPase complex Molecular Function:ATP binding; ATPase activator activity; ATPase activity; ATPase binding; drug binding; potassium ion binding; protein binding; protein C-terminus binding; sodium ion binding; sodium:potassium-exchanging ATPase activity Biological Process: ATP metabolic process; blood coagulation; cardiac muscle contraction; cell adhesion; cellular calcium ion homeostasis; cellular potassium ion homeostasis; cellular sodium ion homeostasis; establishment and/or maintenance of transmembrane electrochemical gradient; leukocyte migration; positive regulation of ATPase activity; positive regulation of defense response to virus by host; potassium ion import; protein stabilization; regulation of gene expression; response to hypoxia; transmembrane transport Disease: Hypertension, Essential |
| NCBI Summary: | The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. [provided by RefSeq, Mar 2010] |
| UniProt Code: | P05026 |
| NCBI GenInfo Identifier: | 114392 |
| NCBI Gene ID: | 481 |
| NCBI Accession: | P05026.1 |
| UniProt Secondary Accession: | P05026,Q5TGZ3, |
| UniProt Related Accession: | P05026 |
| Molecular Weight: | 34,893 Da |
| NCBI Full Name: | Sodium/potassium-transporting ATPase subunit beta-1 |
| NCBI Synonym Full Names: | ATPase Na+/K+ transporting subunit beta 1 |
| NCBI Official Symbol: | ATP1B1�� |
| NCBI Official Synonym Symbols: | ATP1B�� |
| NCBI Protein Information: | sodium/potassium-transporting ATPase subunit beta-1 |
| UniProt Protein Name: | Sodium/potassium-transporting ATPase subunit beta-1 |
| UniProt Synonym Protein Names: | Sodium/potassium-dependent ATPase subunit beta-1 |
| UniProt Gene Name: | ATP1B1�� |
| UniProt Entry Name: | AT1B1_HUMAN |
