Description
| Product Name: | Human ASMT Recombinant Protein |
| Product Code: | RPPB1418 |
| Size: | 20µg |
| Species: | Human |
| Target: | ASMT |
| Synonyms: | HIOMT, HIOMTY, Acetylserotonin O-methyltransferase , Hydroxyindole O-methyltransferase , ASMT. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The ASMT solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) , 1M Urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSSEDQAYR LLNDYANGFM VSQVLFAACE LGVFDLLAEA PGPLDVAAVA AGVRASAHGT ELLLDICVSL KLLKVETRGG KAFYRNTELS SDYLTTVSPT SQCSMLKYMG RTSYRCWGHL ADAVREGRNQ YLETFGVPAE ELFTAIYRSE GERLQFMQAL QEVWSVNGRS VLTAFDLSVF PLMCDLGGDF FKDPLPEADL YILARVLHDW ADGKCSHLLE RIYHTCKPGG GILVIESLLD EDRRGPLLTQ LYSLNMLVQT EGQERTPTHY HMLLSSAGFR DFQFKKTGAI YDAILARK |
ASMT is a member of the methyltransferase superfamily. ASMT participates in melatonin biosynthesis. ASMT Expressed in brain, retina and pineal gland, ASMT utilities to catalyze the final reaction in the synthesis of melatonin, particularly the conversion of S-adenosyl-L-methionine and N-acetylserotonin to S-adenosyl-Lhomocysteine and melatonin.
ASMT Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 318 amino acids (1-298 a.a) and having a molecular mass of 35.3kDa.ASMT is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ASMT: Produces melatonin (N-acetyl-5-methoxytryptamine) from N-acetylserotonin. Belongs to the methyltransferase superfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - tryptophan; EC 2.1.1.4; Methyltransferase Chromosomal Location of Human Ortholog: Xp22.33 and Yp11.2 Cellular Component: cytosol Molecular Function:acetylserotonin O-methyltransferase activity; identical protein binding; O-methyltransferase activity; protein homodimerization activity; S-methyltransferase activity Biological Process: indolalkylamine biosynthetic process; melatonin biosynthetic process; translation |
| NCBI Summary: | This gene belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant in the pineal gland. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2010] |
| UniProt Code: | P46597 |
| NCBI GenInfo Identifier: | 1170276 |
| NCBI Gene ID: | 438 |
| NCBI Accession: | P46597.1 |
| UniProt Secondary Accession: | P46597,Q16598, Q5JQ72, Q5JQ73, B2RC33, |
| UniProt Related Accession: | P46597 |
| Molecular Weight: | Calculated MW: 33kDa/38kDa/41kDaObserved MW: 52kDa |
| NCBI Full Name: | Acetylserotonin O-methyltransferase |
| NCBI Synonym Full Names: | acetylserotonin O-methyltransferase |
| NCBI Official Symbol: | ASMT�� |
| NCBI Official Synonym Symbols: | ASMTY; HIOMT; HIOMTY�� |
| NCBI Protein Information: | acetylserotonin O-methyltransferase |
| UniProt Protein Name: | Acetylserotonin O-methyltransferase |
| UniProt Synonym Protein Names: | Hydroxyindole O-methyltransferase; HIOMT |
| Protein Family: | Acetylserotonin O-methyltransferase |
| UniProt Gene Name: | ASMT�� |
