Description
| Product Name: | Human ASF1B Recombinant Protein |
| Product Code: | RPPB2806 |
| Size: | 20µg |
| Species: | Human |
| Target: | ASF1B |
| Synonyms: | Histone chaperone ASF1B, Anti-silencing function protein 1 homolog B, hAsf1, hAsf1b, CCG1-interacting factor A-II, CIA-II, hCIA-II, ASF1B. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | ASF1B protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.15M NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES EEFDQILDSV LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT YHGQEFIRVG YYVNNEYLNP ELRENPPMKP DFSQLQRNIL ASNPRVTRFH INWDNNMDRL EAIETQDPSL GCGLPLNCTPIKGLGLPGCI PGLLPENSMD CILEHHHHHH |
ASF1 Anti-Silencing Function 1 Homolog B (ASF1B) belongs to the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 protein in yeast. ASF1B is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may have a crucial role in modulating the nucleosome structure of chromatin by guaranteeing a regular supply of histones at sites of nucleosome assembly. ASF1B cooperates with CAF-1 (chromatin assembly factor 1) to stimulate replication-dependent chromatin assembly. ASF1B is highly expressed in the testis and at lower levels in colon, small intestine and thymus. ASF1B is necessary for spermatogenesis.
ASF1B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 210 amino acids (1-202 a.a) and having a molecular mass of 23.4kDa.ASF1B is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ASF1B: Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis. Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with HAT1, NASP, TAF1, TLK1 and TLK2. Highly expressed in testis and at lower levels in colon, small intestine and thymus. Belongs to the ASF1 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 19p13.12 Cellular Component: nuclear chromatin; nucleoplasm; protein complex Molecular Function:histone binding; protein binding Biological Process: cell differentiation; chromatin modification; DNA replication-dependent nucleosome assembly; DNA replication-independent nucleosome assembly; multicellular organismal development; regulation of transcription, DNA-dependent; spermatogenesis; transcription, DNA-dependent |
| NCBI Summary: | This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NVP2 |
| NCBI GenInfo Identifier: | 74734533 |
| NCBI Gene ID: | 55723 |
| NCBI Accession: | Q9NVP2.1 |
| UniProt Secondary Accession: | Q9NVP2,Q53G51, Q9NVZ0, |
| UniProt Related Accession: | Q9NVP2 |
| Molecular Weight: | 22,434 Da |
| NCBI Full Name: | Histone chaperone ASF1B |
| NCBI Synonym Full Names: | anti-silencing function 1B histone chaperone |
| NCBI Official Symbol: | ASF1B�� |
| NCBI Official Synonym Symbols: | CIA-II�� |
| NCBI Protein Information: | histone chaperone ASF1B |
| UniProt Protein Name: | Histone chaperone ASF1B |
| UniProt Synonym Protein Names: | Anti-silencing function protein 1 homolog B; hAsf1; hAsf1b; CCG1-interacting factor A-II; CIA-II; hCIA-II |
| Protein Family: | Histone chaperone |
| UniProt Gene Name: | ASF1B�� |
| UniProt Entry Name: | ASF1B_HUMAN |
