Description
| Product Name: | Human ARHGDIB Recombinant Protein |
| Product Code: | RPPB2782 |
| Size: | 5µg |
| Species: | Human |
| Target: | ARHGDIB |
| Synonyms: | Rho GDP dissociation inhibitor (GDI) beta, Rho GDI 2, Rho-GDI beta, GDIA2, GDID4, Ly-GDI, RAP1GN1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The ARHGDIB solution (1mg/1ml) contains phosphate-buffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMTE KAPEPHVEED DDDELDSKLN YKPPPQKSLK ELQEMDKDDE SLIKYKKTLL GDGPVVTDPK APNVVVTRLT LVCESAPGPI TMDLTGDLEA LKKETIVLKE GSEYRVKIHF KVNRDIVSGL KYVQHTYRTG VKVDKATFMV GSYGPRPEEY EFLTPVEEAP KGMLARGTYH NKSFFTDDDK QDHLSWEWNL SIKKEWTE |
Members of the Rho (or ARH) protein family and other Ras-related small GTP-binding proteins are involved in various cellular procedures, such as cell cytoskeletal organization, secretion, proliferation, and signaling. The GTPBPs are active only in the GTP-bound state. At least 3 types of proteins firmly regulate cycling between the GTP-bound and GDP-bound states: GDP-dissociation inhibitors (GDIs), GTPase-activating proteins (GAPs) and guanine nucleotide-releasing factors (GRFs). ARHGDIB and the other GDIs lower the level of GDP dissociation from Ras-like GTPases.
ARHGDIB Human Recombinant produced in�E. coli is a single polypeptide chain containing 428 amino acids (1-201) and having a molecular mass of 49.4kDa.ARHGDIB is fused to a 227 amino acid GST-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RhoGDI beta: regulates the GDP/GTP exchange reaction of Rho proteins by inhibiting the dissociation of GDP from them, keeping these small GTPases in an inactive state. The GDIs are made up of two domains: a flexible N-terminal domain of about 70 amino acid residues and a folded 134-residue C-terminal domain. Regulates the GTP/GDP cycling of glucose-responsive small GTPases. Its phosphorylation on Y156, S101, and S174 mediates the cycling of Cdc42 and Rac1 to regulate second-phase insulin secretion. Interacts with WNK1. |
| UniProt Protein Details: | Protein type:G protein regulator, misc.; Cell adhesion; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 12p12.3 Cellular Component: cytoskeleton; cytoplasm; cytoplasmic membrane-bound vesicle; cytosol Molecular Function:Rho GDP-dissociation inhibitor activity; GTPase activator activity Biological Process: regulation of small GTPase mediated signal transduction; regulation of catalytic activity; small GTPase mediated signal transduction; multicellular organismal development; immune response; negative regulation of cell adhesion; cell motility; actin cytoskeleton organization and biogenesis; Rho protein signal transduction; positive regulation of GTPase activity |
| NCBI Summary: | Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases (summary by Scherle et al., 1993 [PubMed 8356058]).[supplied by OMIM, Dec 2010] |
| UniProt Code: | P52566 |
| NCBI GenInfo Identifier: | 1707893 |
| NCBI Gene ID: | 397 |
| NCBI Accession: | P52566.3 |
| UniProt Secondary Accession: | P52566,B5BU79, |
| UniProt Related Accession: | P52566 |
| Molecular Weight: | 201 |
| NCBI Full Name: | Rho GDP-dissociation inhibitor 2 |
| NCBI Synonym Full Names: | Rho GDP dissociation inhibitor (GDI) beta |
| NCBI Official Symbol: | ARHGDIB�� |
| NCBI Official Synonym Symbols: | D4; GDIA2; GDID4; LYGDI; Ly-GDI; RAP1GN1; RhoGDI2�� |
| NCBI Protein Information: | rho GDP-dissociation inhibitor 2; Rho GDI 2; rho-GDI beta |
| UniProt Protein Name: | Rho GDP-dissociation inhibitor 2 |
| UniProt Synonym Protein Names: | Ly-GDI; Rho-GDI beta |
| Protein Family: | Rho GDP-dissociation inhibitor |
| UniProt Gene Name: | ARHGDIB�� |
| UniProt Entry Name: | GDIR2_HUMAN |
