Description
| Product Name: | Human ARF3 Recombinant Protein |
| Product Code: | RPPB2774 |
| Size: | 20µg |
| Species: | Human |
| Target: | ARF3 |
| Synonyms: | ADP-ribosylation factor 3, ARF3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | ARF3 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGNIFGNLLK SLIGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLANQLKNK K |
ADP-ribosylation factor 3 (ARF3) belongs to the human ARF gene family. This family encodes small guanine nucleotide-binding proteins which stimulate the ADP-ribosyltransferase activity of cholera toxin and have a role in vesicular trafficking and as activators of phospholipase D. ARF3 functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. ARF3 is involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. The ARF3 gene is comprised of 5 exons and 4 introns.
ARF3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 201 amino acids (1-181 a.a.) and having a molecular mass of 22.8kDa.ARF3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ARF3: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family. |
| UniProt Protein Details: | Protein type:G protein, monomeric, ARF; G protein; G protein, monomeric; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 12q13 Cellular Component: Golgi membrane Molecular Function:GTPase activity Biological Process: phosphatidylinositol biosynthetic process; retrograde vesicle-mediated transport, Golgi to ER |
| NCBI Summary: | ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P61204 |
| NCBI GenInfo Identifier: | 47117657 |
| NCBI Gene ID: | 377 |
| NCBI Accession: | P61204.2 |
| UniProt Secondary Accession: | P61204,P16587, A8K6G8, B7ZB63, |
| UniProt Related Accession: | P61204 |
| Molecular Weight: | 16,145 Da |
| NCBI Full Name: | ADP-ribosylation factor 3 |
| NCBI Synonym Full Names: | ADP ribosylation factor 3 |
| NCBI Official Symbol: | ARF3�� |
| NCBI Protein Information: | ADP-ribosylation factor 3 |
| UniProt Protein Name: | ADP-ribosylation factor 3 |
| Protein Family: | Auxin response factor |
| UniProt Gene Name: | ARF3�� |
| UniProt Entry Name: | ARF3_HUMAN |
