Description
| Product Name: | Human ARF1 Recombinant Protein |
| Product Code: | RPPB2773 |
| Size: | 20µg |
| Species: | Human |
| Target: | ARF1 |
| Synonyms: | ARF-1, ADP-ribosylation factor 1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The ARF1 protein solution (1mg/ml) containing 20mM Tris-HCl pH-8, 1mM DTT, 0.1M NaCl & 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K |
ARF1 is a small guanine nucleotide-binding protein that increases the enzymatic activities of cholera toxin. ARF1 is necessary and ubiquitous in eukaryotes. ARF1 takes part in vesicular transport and functioning via phospholipase D activation. ARF1 is involved in membrane traffic and organelle integrity which are closely tied to their reversible association with membranes and distinct interactions with membrane phospholipids.
ARF1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 201 amino acids (1-181 a.a.) and having a molecular mass of 22.8 kDa. The ARF1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ARF1: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. Belongs to the small GTPase superfamily. Arf family. |
| UniProt Protein Details: | Protein type:G protein; G protein, monomeric, ARF; Motility/polarity/chemotaxis; G protein, monomeric Chromosomal Location of Human Ortholog: 1q42 Cellular Component: Golgi membrane; postsynaptic membrane; neuron projection; sarcomere; focal adhesion; perinuclear region of cytoplasm; postsynaptic density; plasma membrane; cytosol Molecular Function:GTPase activity; protein binding; GTP binding; receptor signaling protein activity Biological Process: protein transport; cellular copper ion homeostasis; COPI coating of Golgi vesicle; viral reproduction; regulation of receptor internalization; small GTPase mediated signal transduction; phospholipid metabolic process; phosphatidylinositol biosynthetic process; retrograde vesicle-mediated transport, Golgi to ER; antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport; regulation of defense response to virus by virus |
| NCBI Summary: | ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P84077 |
| NCBI GenInfo Identifier: | 51316985 |
| NCBI Gene ID: | 375 |
| NCBI Accession: | P84077.2 |
| UniProt Secondary Accession: | P84077,P10947, P32889, |
| UniProt Related Accession: | P84077 |
| Molecular Weight: | 181 |
| NCBI Full Name: | ADP-ribosylation factor 1 |
| NCBI Synonym Full Names: | ADP-ribosylation factor 1 |
| NCBI Official Symbol: | ARF1�� |
| NCBI Protein Information: | ADP-ribosylation factor 1 |
| UniProt Protein Name: | ADP-ribosylation factor 1 |
| Protein Family: | Auxin response factor |
| UniProt Gene Name: | ARF1�� |
| UniProt Entry Name: | ARF1_HUMAN |
![Anti-ARF1 Antibody (CAB19977)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/590x590/products/150770/150551/anti-arf1-antibody-cab19977ko-validated__02815__38587.1750436216.jpg?c=1 "Anti-ARF1 Antibody (CAB19977)[KO Validated]")